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Human embryonic stem cells do not change their X-inactivation status during differentiation

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE88933 | GEO | 2017/01/26

SECONDARY ACCESSION(S): PRJNA349145

REPOSITORIES: GEO

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Human Embryonic Stem Cells Do Not Change Their X Inactivation Status during Differentiation.

Project description: Not available

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Analyses of X-inactivation status in female human induced pluripotent stem cells

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Human embryonic stem cells do not change their X-inactivation status during differentiation [RRBS-Seq]

Project description:Female human ESC-lines can carry active X-chromosomes (Xa) or an XIST-RNA-coated inactive X-chromosome (XiXIST+). Additionally, many ESC lines have abnormal X-chromosomeinactivation (XCI)-states where the Xi no longer expresses XIST-RNA and has transcriptionally active regions (eroded Xi=Xe). The fate of each XCI-state upon differentiation is unclear because individual lines often contain a mixture of XCI-states. Here, we established homogeneous XiXa, XeXa, and XaXa ESC-lines. Employing RNA-FISH, RNA-sequencing and DNA methylation analyses, we found that these lines were unable to initiate XIST-expression and X-chromosome-wide silencing upon differentiation indicating that the ESC XCI-state is maintained in differentiated cells. Consequently, differentiated XeXa and XaXa cells displayed higher levels of X-linked gene-expression than XiXa cells. Although global transcriptional compensation between X-chromosomes and autosomes is not required for female ESC-differentiation, the degree of X-chromosome-silencing influences differentiation efficiencies. Our data suggest that the XiXIST+Xa state is inherent to human ESCs and that all other XCI-states, including XaXa, are abnormal and arise during ESC-derivation or maintenance.

2017-01-26 | GSE88932 | GEO

Human embryonic stem cells do not change their X-inactivation status during differentiation [RNA-Seq]

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Pluripotent mouse Embryonic Stem Cells and during Retinoic Acid-induced differentiation

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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KDM5B focuses H3K4 methylation near promoters and enhancers during embryonic stem cell self-renewal and differentiation

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Nucleosome positioning changes during human embryonic stem cell differentiation

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-05-16 | GSE46467 | GEO

Enhancer Decommissioning by LSD1 During Embryonic Stem Cell Differentiation

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-01-03 | GSE27844 | GEO

Analyses of X-inactivation status in female human induced pluripotent stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2013-02-06 | GSE44066 | GEO

Human embryonic stem cells do not change their X-inactivation status during differentiation

Project description:Human embryonic stem cells do not change their X-inactivation status during differentiation

| PRJNA349145 | ENA

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