Genomics

Dataset Information

99

Integrative analysis of reprogramming human fibroblast cells to naïve pluripotency [RRBS]


ABSTRACT: Derivation of human naïve cells in the ground state of pluripotency provides promising avenues for developmental studies and therapeutic manipulations. However, the molecular mechanisms involved in establishment and maintenance of human naïve pluripotency remain poorly understood. Using the human inducible reprogramming system together with 5iLAF naïve induction strategy, we performed integrative analysis across the timeline from human fibroblasts to naïve iPSCs, which reveals ordered expression waves of gene networks sharing signatures with embryonic development process from post-implantation to pre-implantation stages. We also observed a significant transient re-activation of transcripts with 8-cell-stage-like characteristics in late naïve reprogramming stages. Moreover, combined quantitative proteomics analysis with transcriptional dynamics during naïve pluripotency induction, we identified ALPPL2 as the specific surface marker for human naïve pluripotency. Altogether, our study deepens the understanding of molecular mechanisms of human naïve pluripotency. Overall design: RRBS profiles of transitioning cells at different time points in the naïve/primed reprogramming process were performed by deep sequencing.

INSTRUMENT(S): Illumina HiSeq 2000 (Homo sapiens)

SUBMITTER: Shaorong Gao  

PROVIDER: GSE89060 | GEO | 2018-03-03

REPOSITORIES: GEO

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Publications

Unique molecular events during reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) at naïve state.

Wang Yixuan Y   Zhao Chengchen C   Hou Zhenzhen Z   Yang Yuanyuan Y   Bi Yan Y   Wang Hong H   Zhang Yong Y   Gao Shaorong S  

eLife 20180130


Derivation of human naïve cells in the ground state of pluripotency provides promising avenues for developmental biology studies and therapeutic manipulations. However, the molecular mechanisms involved in the establishment and maintenance of human naïve pluripotency remain poorly understood. Using the human inducible reprogramming system together with the 5iLAF naïve induction strategy, integrative analysis of transcriptional and epigenetic dynamics across the transition from human fibroblasts  ...[more]

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