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Gene expression during metabolism of polysaccharides and sugars by Alicyclobacillus acidocaldarius

ABSTRACT: The purpose of this study was to characterize carbon metabolism and gene regulation in Alicyclobacillus acidocaldarius during growth on wheat arabinoxylan and the effect of pentose and hexose sugars on gene expression Chemostat studies and global transcriptome analysis were used to accomplish this goal.

ORGANISM(S): Alicyclobacillus acidocaldarius

PROVIDER: GSE89078 | GEO | 2017/01/01

SECONDARY ACCESSION(S): PRJNA350247

REPOSITORIES: GEO

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Gene expression during metabolism of sugars by Alicyclobacillus acidocaldarius

Project description:The purpose of this study was to characterize carbon metabolism and gene regulation in Alicyclobacillus acidocaldarius during growth on monosaccharides in an effort to determine whether carbon catabolite repression was active. Chemostat studies and global transcriptome analysis were used to accomplish this goal.

2016-08-31 | GSE84032 | GEO

Absence of di-auxie during simultaneous utilization of glucose and xylose by Sulfolobus acidocaldarius

Project description:Sulfolobus acidocaldarius has been previously reported to grow on a broad range of sugars, but there is limited information on the ability of the organism to metabolize multiple sugars simultaneously. We report here the ability of S. acidocaldarius to utilize glucose and xylose simultaneously without di-auxie effect. The organism utilized a mixture of 1 g/L glucose and 1 g/L xylose with a growth rate of 0.079 h-1 compared to 0.074 h-1 and 0.22 h-1 when the organism was grown on xylose 2 g/L and 2 g/L glucose respectively as sole carbon sources. An increase in xylose concentration to 2 and 4 g/L in a medium containing 1 g/L glucose resulted in a growth rate of 0.082 and 0.085 h-1. However, increasing glucose concentration by 2 and 4 g/L when xylose concentration was maintained at 1 g/L decreased the growth rate to 0.062 and 0.052 h-1 respectively. S. acidocaldarius appeared to be utilizing the sugars at a rate roughly proportional to their concentration in the medium, resulting in complete utilization of these sugars at same time. The organism did not show preference for either glucose or xylose when it was grown on both sugars. Similar results were obtained with a combination of glucose, arabinose and galactose. These observations strongly suggest that S. acidocaldarius does not regulate utilization of the sugars tested herein using carbon catabolite repression (CCR) commonly found in most bacteria. The mechanism by which the organism utilized a mixture of sugar is yet to be elucidated. However, we were able to identify genes encoding for the putative glucose ABC transporters; but the putative xylose transporter was not identified. A study of S. acidocaldarius grown in glucose, xylose, or the two carbon sources combined in mid-log. Analyzed on Nimblegen 4-plex S. acidocaldarius DSM 639 array weblink: http://microbesonline.org/cgi-bin/microarray/viewExp.cgi?expId=1723

2010-07-31 | E-GEOD-21695 | biostudies-arrayexpress

Absence of di-auxie during simultaneous utilization of glucose and xylose by Sulfolobus acidocaldarius

2010-07-31 | GSE21695 | GEO

Alicyclobacillus acidocaldarius

Project description:Gene expression during metabolism of sugars by Alicyclobacillus acidocaldarius

| PRJNA327845 | ENA

Alicyclobacillus acidocaldarius

Project description:Gene expression during metabolism of polysaccharides and sugars by Alicyclobacillus acidocaldarius

| PRJNA350247 | ENA

Transcriptome analysis of Sulfolobus acidocaldarius DSM 639 under sugar-free and sugar-rich conditions

Project description:Sulfolobus acidocaldarius is an obligate aerobe that grows in hot and acidic environments. S. acidocaldarius have been reported to grow on a variety of organic compounds as carbon and energy sources. However, little is known about systemic elucidation of carbon utilization for biomass formation and energy metabolism in S. acidocaldarius. In this analysis, the effect of glucose on genome-wide transcriptional profiling in S. acidocaldarius DSM 639 was investigated by RNA-Seq technology.

2020-11-15 | GSE107083 | GEO

Alicyclobacillus acidocaldarius subsp. acidocaldarius HSU9

Project description:Alicyclobacillus acidocaldarius subsp. acidocaldarius HSU9 Genome sequencing

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Sulfolobus acidocaldarius phosphoproteome

Project description:Shotgun phosphoproteome of Sulfolobus acidocaldarius using PAciFIC technique. Briefly, proteins were denatured, alkylated, trypsine digestion, SCX separation Fractionated peptides were analysed on Bruker HCTultra. Data processing and bioinformatics: data from mass spectrometry were then extracted to mgf format using Bruker Data Analysis V4.0 with a MRM script, these were then were searched against the Sulfolobus acidocaldarius database (containing 2223 proteins) downloaded from NCBI in March 2010 using Phenyx V 2.6 (Genebio, Geneva). The searches were performed using parameters as follows: carbamidomethylation of cysteine (fixed modification), oxidation of methionine (variation), and phosphorylation of serine, tyrosine, threonine (variation), trypsin with 2 missed cleavages. Furthermore, other parameters such as parent, MS/MS, tolerances were set at 2.0 and 0.8 Da, respectively, whilst minimum peptide length, z-score, p-value and AC score were set at 5, 5.5, 10-5, and 5.5 respectively.

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Genome-wide interaction map of YtrA transcription factor in S. Acidocaldarius

Project description:We report the characterization of GntR-like regulator in the Archaeon Sulfolobus acidocaldarius by looking at the genome wide interaction map of the protein in vivo

2019-09-26 | GSE129461 | GEO

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Project description:Alicyclobacillus acidocaldarius overview

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