Transcriptomics

Dataset Information

36

Profiling differentially expressed earthworm genes in response to TNT, RDX, or TNT+RDX exposure.


ABSTRACT: To understand molecular mechanisms of the joint effects of 2,4,6-trinitrotoluene (TNT) and hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), both widely used ordnance compounds, we constructed a microarray consisting of 4,032 cDNA isolated from the earthworm Eisenia fetida using the suppressive subtractive hybridization technique. Worms were exposed to TNT-, RDX-, or TNT+RDX-spiked soil for 28 days (TNT 50 mg/kg, RDX 30 mg/kg). Keywords: Combined toxicity of TNT and RDX to earthworm (Eisenia fetida) Overall design: We analyzed 40 arrays for 4 treatments (control, TNT 50ppm, RDX 30ppm, TNT 50ppm + RDX 30ppm) with 5 biological replicates per treatment using an interwoven loop design.

INSTRUMENT(S): Earthworm 4K cDNA array version 1.1

ORGANISM(S): Eisenia fetida  

SUBMITTER: Ping Gong  

PROVIDER: GSE8909 | GEO |

SECONDARY ACCESSION(S): PRJNA102303

REPOSITORIES: GEO

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