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The influence of recurrent EBV BRLF1 expression on genetic copy number alterations

ABSTRACT: The EBV BRLF1 expression may exert an influence on genome instability. To test this speculation, the long-term experiment of recurrent BRLF1 expression was performed. The NPC cells (TW01-TetER, doxycycline inducible BRLF1 expressing cell line) were used. Experimental samples were derived from different passages of the NPC cells without or with BRLF1 expression. Array CGH (aCGH) was carried out to investigate the copy-number aberrations in response for different frequencies of BRLF1 expression. Compared to P1 as a common reference, a dramatic increase of aberrations was observed in R15 especially on chromosomes 3, 4, 5, 6, 7, 11, 12, 13, 14, 15, 16, 17 and 21. However, relatively few aberrations were detected in R1. Under long-term cell culture, P15 was not observed obviously changed in terms of aberrations. According to the results, recurrent expression of BRLF1 may be critical in inducing aggravated genome instability in NPC cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE89105 | GEO | 2020/04/24

REPOSITORIES: GEO

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