Genomics

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Comparison of gene expression changes following knockdown of KMT2A and MSK1 in mouse embryonic fibroblasts


ABSTRACT: The KMT2A/MLL1 lysine methyltransferase complex is an epigenetic regulator of selected developmental genes, in part through the SET-domain-catalyzed methylation of H3K4. It is essential for normal embryonic development and haematopoiesis and frequently mutated in cancer. The catalytic properties and targeting of KMT2A/MLL1 depend on the proteins with which it complexes and the post-translational protein modifications which some of these proteins put in place, though detailed mechanisms remain unclear. We have shown that KMT2A/MLL1 (both native and FLAG-tagged) and Msk1 (RPS6KA5), co-immunoprecipitated in various cell types. This experiment showed that the great majority of genes whose activity changed on KTM2A/MLL1 knockdown responded comparably to Msk1 knockdown.

ORGANISM(S): Mus musculus

PROVIDER: GSE89141 | GEO | 2016/10/26

SECONDARY ACCESSION(S): PRJNA350585

REPOSITORIES: GEO

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