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HCoV-229E infection of the human HuH7 cell line [Experiment A]

ABSTRACT: The aim of the experiment is to identify changes in host gene expression upon infection of HuH7 human hepatoma cells with Human corona virus-229E (HCoV-229E) compared to mock-infected cells. All experiments were performed at 33°C which is required for sufficient viral entry/replication.

ORGANISM(S): Homo sapiens

PROVIDER: GSE89160 | GEO | 2017/03/15

SECONDARY ACCESSION(S): PRJNA350586

REPOSITORIES: GEO

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HCoV-229E infection of the human HuH7 cell line [Experiment B]

Project description:The aim of the experiment is to identify changes in host gene expression upon infection of Huh7 hepatoma carcinoma cells with human coronavirus HCoV-229E as compared to uninfected cells and in comparison to cells stimulated by IL-1alpha (10ng/ml) for 1h. All experiments have been performed at 33°C which is required for sufficient viral entry/replication. Also included are two samples which have been treated with a heat inactivated coronavirus (56°C for 5min).

2017-03-15 | GSE89166 | GEO

Time-course of HCoV-229E infection of A549 cells

Project description:The aim of the experiment is to identify kinetic changes in host gene expression upon infection of A549 lung epithelial carcinoma cells with human corona virus HCoV-229E as compared to unifected cells. Negativ controls include infections with heat-inactivated virus. Positive controls include treatment of cells with human IL-1alpha (10ng/ml) for 1h. All experiments have been performed at 33°C which is required for sufficient viral entry/replication.

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Transcriptomic profiling of human corona virus (HCoV)-229E -infected human cells

Project description:Human coronaviruses (HCoVs) cause mild to severe respiratory infection. Most of the common cold illnesses are caused by one of four HCoVs, namely HCoV-229E, HCoV-NL63, HCoV-HKU1 and HCoV-OC43. Several studies have applied global transcriptomic methods to understand host responses to HCoV infection, with most studies focusing on the pandemic severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome CoV (MERS-CoV) and the newly emerging SARS-CoV-2. In this study, Next Generation Sequencing was used to gain new insights into cellular transcriptomic changes elicited by alphacoronavirus HCoV-229E. HCoV-229E-infected MRC5 cells showed marked downregulation of superpathway of cholesterol biosynthesis and eIF2 signaling pathways. Moreover, upregulation of cyclins, cell cycle control of chromosomal replication, and role of BRCA1 in DNA damage response, alongside downregulation of the cell cycle G1/S checkpoint, suggest that HCoV-229E favors S phase for viral infection. Intriguingly, more than 80% of key factors of cell innate immunity, interferon-stimulated genes (ISGs) and other transcripts of early antiviral response genes were downregulated early in HCoV-229E infection. This study will enhance our understanding of commonly circulating HCoVs and hopefully provide critical information about still-emerging coronaviruses.

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Transcriptomic profiling of human corona virus (HCoV)-229E -infected human cells

Project description:Transcriptomic profiling of human corona virus (HCoV)-229E -infected human cells

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ChIP-seq analysis of coronavirus-infected human cells

Project description:The aim of the experiment is to identify chromatin and transcription factor binding dynamics in host gene expression upon infection of human Huh7 hepatoma carcinoma cells with human coronavirus HCoV-229E as compared to uninfected cells and in comparison to cells stimulated by IL-1alpha (10ng/ml) for 1h. All experiments have been performed at 33°C which is required for sufficient viral entry/replication.

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ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection II

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N-Tera2 differentiated human neuronal model: Mock infected vs HCoV-OC43 infected

Project description:Transcriptional profiling of N-Tera2 differentiated human neuronal cells, comparing control uninfected cells to HCoV-OC43 infected cells at 24, 48 and 72 hour post-infection Keywords: Cell response to viral infection Two-condition experiment, N-Tera2 differentiated human neuronal cell mock infected vs. N-Tera2 differentiated human neuronal cell HCoV-OC43 infected at 24, 48 and 72 hours. Biological replicates: 2 at each time-course point. Technical replicate: 2 dye-swap at each time-point. 2 arrays hybridized with mock(cy3) vs infected(cy5) and 2 array with infected(cy3) vs mock(cy5).

2008-12-10 | E-GEOD-13879 | biostudies-arrayexpress

Homo sapiens

Project description:HCoV-229E infection of the human HuH7 cell line [Experiment B]

| PRJNA350589 | ENA

Homo sapiens

Project description:HCoV-229E infection of the human HuH7 cell line [Experiment A]

| PRJNA350586 | ENA