Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide transcriptomic and epigenomic study of DHA in rat hypothalamus and hippocampus [RRBS]

ABSTRACT: To investigate the effects of DHA (an omega-3 fatty acid) on brain dysfunction and metabolic disorders by sequencing the transcriptome (RNA-Seq) and epigenome (RRBS) in the hypothalamus and hippocampus of male SD rats.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE89175 | GEO | 2018/12/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Genome-wide transcriptomic and epigenomic study of DHA in rat hypothalamus and hippocampus [RNA-seq]

Project description:To investigate the effects of DHA (an omega-3 fatty acid) on brain dysfunction and metabolic disorders by sequencing the transcriptome (RNA-Seq) and epigenome (RRBS) in the hypothalamus and hippocampus of male SD rats.

2018-12-31 | GSE89173 | GEO

DNA methylation profile for male SD Rats upon fructose and DHA treatment by RRBS

Project description:To investigate the beneficial effects of DHA (omega-3) on affecting fructose induced brain dysfunction and metabolic disorders by sequencing the methylome (RRBS) in the hypothalamus and hippocampus of male SD rats.

2016-10-19 | GSE64816 | GEO

Gene expression profile for male SD Rats upon fructose and DHA treatment by RNA-Seq

Project description:To investigate the beneficial effects of DHA (omega-3) on affecting fructose induced brain dysfunction and metabolic disorders by sequencing the transcripome (RNA-Seq) in the hypothalamus and hippocampus of male SD rats.

2016-07-13 | GSE64815 | GEO

Gene Expressoin Profile in the Hypothalamus of Male Bgn_KO and Bgn_KO Mice fed with DHA at 13 Weeks Old

Project description:To investigate the effects of DHA (omega-3) treatment on affecting brain dysfunction and metabolic disorders by profiling the transcripome in the hypothalamus of male Bgn_KO mice (with and without DHA) at 13 weeks old.

2018-12-31 | GSE64807 | GEO

Genome-wide transcriptomic and epigenomic study of DHA in rat hypothalamus and hippocampus

Project description:Genome-wide transcriptomic and epigenomic study of DHA in rat hypothalamus and hippocampus

| PRJNA352566 | ENA

LDLR-/- mice aorte: Control vs DHA

Project description:The omega-3 fatty acid docosahexaenoic acid (DHA) has potent anti-atherogenic properties but its mechanisms of action at the vascular level remain poorly explored. Knowing the broad range of molecular targets of omega-3 fatty acids, microarray analysis was used to open-mindedly evaluate the effects of DHA on aorta gene expression in LDLR-/- mice and better understand its local anti-atherogenic action . Mice were fed an atherogenic diet and received daily oral gavages with oils rich in oleic acid or DHA. Bioinformatics analysis of microarray data first identified inflammation and innate immunity as processes the most affected by DHA supplementation within aorta. More precisely, several down-regulated genes were associated with the inflammatory functions of macrophages (e.g. CCL5, CCR7), cell movement (e.g. ICAM-2, SELP, PECAM-1), and the major histocompatibility complex (e.g. HLA-DQA1, HLA-DRB1). Interestingly, the expression of several genes were identified as specifc biomarkers of macrophage polarization and their changes suggested a preferential orientation towards a M2 reparative phenotype. This observation was supported by the upstream regulator analysis highlighting the involvment of three main regulators of macrophage polarization, namely PPARM-NM-3 (z-score=2.367, p=1.50x10-13), INFM-NM-3 (z-score=-2.797, p=2.81x10-14) and NFM-NM-:B (z-score=2.360, p=6.32x10-9). Moreover, immunohistological analysis of aortic root revealed an increased abundance of Arg1 (+111%, p=0.01), a specific biomarker of M2 macrophage.The present study showed for the first time that DHA supplementation during atherogenesis is associated with protective modulation of inflammation and innate immunity pathways within aorta putatively through the orientation of plaque macrophages towards a M2 reparative phenotype. Mice (LDLR-/-) Aorta samples. 2 groups: Control (K), DHA (C). Biological replicates=8. Dye switch.

2015-01-10 | E-GEOD-59661 | biostudies-arrayexpress

LDLR-/- mice aorte: Control vs DHA

Project description:The omega-3 fatty acid docosahexaenoic acid (DHA) has potent anti-atherogenic properties but its mechanisms of action at the vascular level remain poorly explored. Knowing the broad range of molecular targets of omega-3 fatty acids, microarray analysis was used to open-mindedly evaluate the effects of DHA on aorta gene expression in LDLR-/- mice and better understand its local anti-atherogenic action . Mice were fed an atherogenic diet and received daily oral gavages with oils rich in oleic acid or DHA. Bioinformatics analysis of microarray data first identified inflammation and innate immunity as processes the most affected by DHA supplementation within aorta. More precisely, several down-regulated genes were associated with the inflammatory functions of macrophages (e.g. CCL5, CCR7), cell movement (e.g. ICAM-2, SELP, PECAM-1), and the major histocompatibility complex (e.g. HLA-DQA1, HLA-DRB1). Interestingly, the expression of several genes were identified as specifc biomarkers of macrophage polarization and their changes suggested a preferential orientation towards a M2 reparative phenotype. This observation was supported by the upstream regulator analysis highlighting the involvment of three main regulators of macrophage polarization, namely PPARγ (z-score=2.367, p=1.50x10-13), INFγ (z-score=-2.797, p=2.81x10-14) and NFκB (z-score=2.360, p=6.32x10-9). Moreover, immunohistological analysis of aortic root revealed an increased abundance of Arg1 (+111%, p=0.01), a specific biomarker of M2 macrophage.The present study showed for the first time that DHA supplementation during atherogenesis is associated with protective modulation of inflammation and innate immunity pathways within aorta putatively through the orientation of plaque macrophages towards a M2 reparative phenotype.

2015-01-10 | GSE59661 | GEO

Effect of DHA enriched fish oil on human placental cells

Project description:The omega-3 long chain poly-unsaturated fatty acid (n-3 LCPUFA) docosahexaenoic acid (DHA) plays a central role in fetal and neonatal development in humans, in particular the development of the brain and nervous system, and this has led to numerous studies focussed on determining the effect of exposure to an increased supply of DHA on pregnancy and neonatal outcomes. The aim of this study was to determine the impact of treatment with a DHA-enriched fish-oil emulsion on fatty acid composition, proliferation rate and gene expression in human placental HTR8/SVneo cells in vitro.

2014-12-31 | GSE50945 | GEO

Genome-wide transcriptomic and epigenomic study of DHA in rat hypothalamus and hippocampus

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-12-31 | GSE89176 | GEO

DNA methylation profile for male SD Rats upon fructose treatment by RRBS

Project description:DNA methylation profile for male SD Rats upon fructose treatment by RRBS

2016-10-19 | GSE59893 | GEO