Genomics

Dataset Information

51

Transcriptomic Regulation Of Scion/Rootstock Harmony And Drought Response In The Roots Of Grapevine Rootstock


ABSTRACT: Grafting is a well-established practice for grapevine to facilitate propagation of productive and tolerant cultivars against several stress factors. It is also considered to be a suitable method for studying molecular aspects of root-to-shoot and/or shoot-to-root signaling events. So far, controlling only effect of rootstock over scion was investigated and root-to-shoot transcriptomic alterations were fallowed on the scions or graft interfaces. The objective of this study was to investigate transcriptomic and physiological influence of scion on the rootstock under drought stress. Therefore, drought tolerant 110R rootstock were firstly grafted with sultana seedless and tested under drought stress with its non-grafted counterpart. The results of treatment indicated that grafted 110R performed the highest root elongation under drought. We carried out a microarray based transcriptome analysis on the roots of grafted and non-grafted 110R to explain this drought derived interaction through scion-to-rootstock. The highest expression increase under drought was recorded for sugar (SWEET) and nitrate or di/tri-peptide (NRT1/ PTR FAMILY) transporter proteins. Expression level of these genes was more highly increased in grafted 110R than its non-grafted counterpart. This situation indicated their potential role in drought tolerance and scion/rootstock harmony. Overexpression of these transporters attributed to increased amount of released nutrient and nitrogen source from abscised leaves of sultana seedless under drought. Remobilization of these rich sources was suggested to chance transcriptomic response of rootstocks and enabled much better growth in grafted 110R. Other transcripts annotated to cell wall modification enzymes (chitinases), osmoregulator proteins (dehydrins, proline-glycine rich proteins) and secondary metabolites (stilbene synthase) were also more highly induced in grafted 110R. This is the first report indicating transcriptomic influence of scion on the grapevine rootstocks and representing the genes responsible in scion/rootstock harmony and drought tolerance. Overall design: Total RNA used in the microarray experiment was isolated from the roots of two grafted and non-grafted 110R rootstock, which is known to be tolerant to drought stress, grown under well watered and drought treated conditions. RNA isolation was made according to Lithium chloride precipitation method described in Chang et al. (1993). These two isolated RNAs (biological replicates) for grafted and non-grafted plants loaded onto two Affymetrix Vitis Genome arrays (technical replicates). Totally, 8 GeneChips (2 rootstock/scion combination x 2 different water availability x 2 biological replicates) were used for transcriptional analysis.

INSTRUMENT(S): [Vitis_Vinifera] Affymetrix Vitis vinifera (Grape) Genome Array

SUBMITTER: KUBILAY YILDIRIM  

PROVIDER: GSE89185 | GEO | 2017-12-31

SECONDARY ACCESSION(S): PRJNA350548

REPOSITORIES: GEO

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