Transcriptomics,Genomics

Dataset Information

33

Expression data from Saccharomyces cerevisiae with eEF1A overexpression


ABSTRACT: The canonical role of eEF1A is to deliver the aminoacyl tRNA to the ribosome, we have used the yeast model system to investigate further roles for this protein. We used microarray to study the transcriptomic effects of elevated levels of eEF1A on yeast cells during log phase growth Overall design: Yeast cells were grown to log phase, the RNA extracted and hybridised to affymetrix arrays.

INSTRUMENT(S): [Yeast_2] Affymetrix Yeast Genome 2.0 Array

SUBMITTER: Daniel Tarrant 

PROVIDER: GSE89286 | GEO | 2016-10-29

SECONDARY ACCESSION(S): PRJNA351243

REPOSITORIES: GEO

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Publications

Inappropriate expression of the translation elongation factor 1A disrupts genome stability and metabolism.

Tarrant Daniel J DJ   Stirpe Mariarita M   Rowe Michelle M   Howard Mark J MJ   von der Haar Tobias T   Gourlay Campbell W CW  

Journal of cell science 20161102 24


The translation elongation factor eEF1A is one of the most abundant proteins found within cells, and its role within protein synthesis is well documented. Levels of eEF1A are tightly controlled, with inappropriate expression linked to oncogenesis. However, the mechanisms by which increased eEF1A expression alters cell behaviour are unknown. Our analyses in yeast suggest that elevation of eEF1A levels leads to stabilisation of the spindle pole body and changes in nuclear organisation. Elevation o  ...[more]

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