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RNA-seq analysis of hypertension induced by a high calcium intake in Chicken broiler kidney under varied calcium intake

ABSTRACT: Chicken (Gallus gallus) were first domesticated from a wild form called red jungle fowl that still runs wild in most of southeast Asia. After then the grey jungle fowl (G. sonneratii) was hybridized, which was occurred probably about 8,000 years ago, and domestic chickens have been selected to have ideal economic traits such as a meat and a laying breed. In these breeds, a meat breed such as broiler is the most distributed in poultry industry. In addition, calcium is essential for normal cellular function and blood coagulation. However, it caused the hypocalcemia and the hypercalcemia if calcium intake was less and more than adequate calcium intake such as hypocalcemia and hypercalcemia, which is related to hypertension and blood pressure. So, we generated RNA-seq data from nine chicken broilers according to calcium intake (0.8, 1.0 and 1.2percent) for gene expression profiling. As a result, we identified differentially expressed genes (DEGs) using cufflinks (128 DEGs between 0.8 versus 1.0 percent, 141 DEGs between 0.8 versus 1.2 percent and 103 DEGs between 1.0 versus 1.2 percent), also 12 DEGs were identified by generalized linear model (GLM) within edgeR. We identified that these DEGs were related to hypertension and blood pressure through the KEGG pathway enrichment, the co-occurrence and the protein/protein interaction (PPI) network analysis. In summary, the objective of this study was to investigate the influence of increasing calcium intake in chicken broilers kidney. Therefore, we suggested that higher calcium intake than adequate in chicken broilers can caused the hypertension and high blood pressure.

ORGANISM(S): Gallus gallus

PROVIDER: GSE89544 | GEO | 2017/11/01

SECONDARY ACCESSION(S): PRJNA352522

REPOSITORIES: GEO

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Project description:Chromosomal structural variation can cause alterations in gene dosage and gene regulation between genomes. Structural variants producing a change in the number of copies of a genomic region are termed copy number variants (CNVs). CNVs have been demonstrated to have causative effects on both Mendelian and complex traits, including susceptibility to infectious diseases. We are interested in mapping CNVs to domesticated chicken breeds to help determine structural variation between genomes that influences economically important traits. For this study, Fayoumi, Leghorn, Line A broiler and Line B broiler chicken were chosen. Fayoumi and Leghorn chickens were selected as these two breeds harbor different responses certain pathogens like Avian Influenza Virus and coccidiosis; Broiler Line A and Line B indivduals were chosen as they harbor different intestinal colonization loads to the bacterium Campylobacter jejuni. Campylobacter genetic Line A and genetic Line B are from a commercial producer have been previously described as either resistant (Line A) or susceptible (Line B). Highly inbred chicken lines Fayoumi M15.2 (n=6) and Leghorn GHs6 (n=6) and broilers from Line A (n=24 individuals in pools of 4) and Line B (n=24 individuals in pools of 4)were subjected to array Comparative Genomic Hybridization (aCGH). Each sample was normalized to a Red Jungle Fowl reference. CNVs for each individual and between lines were determined. The major goal of this study was to discover and characterize CNVs in chickens to further narrow in on Quantitative Trait Loci (QTLs) affecting disease response. For the test DNA in Fayoumi and Leghorn, samples from 6 inbred Fayoumi and 6 inbred Leghorn individuals were used; For the test DNA in the Campylobacter genetic lines, samples from 24 individual broilers of Line A (in pools of 4) and 24 individual broilers of Line B (in pools of 4) were used. For the reference DNA, Red Jungle Fowl line UCD001 was used from a self-self hybridization.

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RNA-seq analysis of hypertension induced by a high calcium intake in Chicken broiler kidney under varied calcium intake

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