Genomics

Dataset Information

152

Cbx3 Maintains Lineage Specificity During Neural Differentiation [ChIP-seq]


ABSTRACT: Cbx3 (HP1γ) that is a member of the heterochromatin protein 1 familiy enriched in gene bodies of pluripotent ESCs but promoters of differentiated pre-iPSCs. To determine whether Cbx3 becomes enriched at promoters to influence gene regulation during differentiation, we converted ESCs to NPCs in monolayer culuture and performed genomewide ChIP-seq of Cbx3. Our results shows that Cbx3 enriches at the promoters of genes upon differentiation of ESCs to NPCs and stablizes the binding of Mediator subunit Med26 to pre-initiation complex (PIC) in NPCs. Overall design: Examine the enrichment of Cbx3 and Pol II in wild-type ESC-derived NPCs, and the enrichment of Med26, Cdk8 and Pol II in NPC transfected with control siRNA targeting luciferase or siRNA mediated knockdown of Cbx3 by ChIP-seq analysis.

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

SUBMITTER: Siavash K Kurdistani  

PROVIDER: GSE89573 | GEO | 2017-03-27

SECONDARY ACCESSION(S): PRJNA352607

REPOSITORIES: GEO

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Publications


Chromobox homolog 3 (Cbx3/heterochromatin protein 1γ [HP1γ]) stimulates cell differentiation, but its mechanism is unknown. We found that Cbx3 binds to gene promoters upon differentiation of murine embryonic stem cells (ESCs) to neural progenitor cells (NPCs) and recruits the Mediator subunit Med26. RNAi knockdown of either Cbx3 or Med26 inhibits neural differentiation while up-regulating genes involved in mesodermal lineage decisions. Thus, Cbx3 and Med26 together ensure the fidelity of lineage  ...[more]

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