Dataset Information


RNA-sequencing of B cells in the absence of Moz or c-Myb

ABSTRACT: Humoral responses of mice specifically deleted for Moz (a histone acetyltransferase) or c-Myb (a transcription factor) in B cells were aberrant. RNA-sequencing analysis was performed to assess gene expression differences compared to wild-type controls in germinal center B cells or plasmablasts. Overall design: Moz f/f Aicda1-Cre, Aicda1-Cre, Myb f/f Cd23-Cre, Mybf/f (no cre) mice were immunized with NP-KLH precipitated in alum and germinal center B cells were sort-purified. Secondary plasmablasts were sort-purified from immunized mice boosted with NP-KLH in PBS (Myb experiment). Two independent experiments were conducted.

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

SUBMITTER: Gordon K Smyth  

PROVIDER: GSE89732 | GEO | 2017-03-07



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Regulation of germinal center responses and B-cell memory by the chromatin modifier MOZ.

Good-Jacobson Kim L KL   Chen Yunshun Y   Voss Anne K AK   Smyth Gordon K GK   Thomas Tim T   Tarlinton David D  

Proceedings of the National Academy of Sciences of the United States of America 20140616 26

Memory B cells and long-lived bone marrow-resident plasma cells maintain humoral immunity. Little is known about the intrinsic mechanisms that are essential for forming memory B cells or endowing them with the ability to rapidly differentiate upon reexposure while maintaining the population over time. Histone modifications have been shown to regulate lymphocyte development, but their role in regulating differentiation and maintenance of B-cell subsets during an immune response is unclear. Using  ...[more]

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