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Time-series analysis of transcription factor knockdown in Drosophila S2R+ cells using RNA-Seq.

ABSTRACT: To understand how RNAi depletion of transcription factors impact the Drosophila transcriptome, we performed selective knockdown of 46 transcription factors in Drosophila S2R+ cell line. We treated cells with long double strand RNAs by bathing for 1 to 3 days. We purified polyA+ RNA and prepared strand-specific RNA-Seq libraries. We quantitatively measured transcriptomic responses using a time series analysis.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE89753 | GEO | 2017/04/26

SECONDARY ACCESSION(S): PRJNA353097

REPOSITORIES: GEO

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Gene expression profiling of Drosophila S2R+ cells following RNAi-mediated knockdown of transcription factors

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ChIP-seq using a Dp1 antibody in Drosophila S2R+ cells

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