Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide map of HAEC chromatin landscape under resting and TNFa, IL1b, and OxPAPC stimulation, with corresponding transcription factor binding and RNA expression

ABSTRACT: We report the dynamic enhancer landscape of HAEC at baseline and under inflammatory stimulation, and also show the cistromes of ETS, AP-1 as well as key signal dependent transcription factors, with corresponding gene expression. We also describe the inflammatory transcriptional profile caused by inhibition of the ETS factor ERG with siRNA.

ORGANISM(S): Homo sapiens

PROVIDER: GSE89970 | GEO | 2017/06/06

SECONDARY ACCESSION(S): PRJNA354352

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Microbiota HAEC

Project description:Microbiota HAEC

| PRJEB55092 | ENA

Effects of model chylomicron remants on gene expresssion in human aortic endothelial cells (HAEC)

Project description:Global gene experssion study of the HAEC transcriptional response to artificial chlyomicron remnant-like particles (A-CRLPs) prepared with triglycerides extracted from four natural dietary oils: fish, DHASCO, corn and palm oils. We hypothesised that A-CRLPs could differentially regulate HAEC gene expression according to thier triglyceride content. These data provide an important starting point for investigations into the effects of A-CRLPs on endothelial cells, particulary genes involved in redox balance and inflammatory processes.

2016-05-19 | GSE80067 | GEO

Effects of model chylomicron remants on gene expresssion in human aortic endothelial cells (HAEC)

2016-05-19 | E-GEOD-80067 | biostudies-arrayexpress

Evidence for the importance of OxPAPC interaction with cysteines in regulating endothelial cell function

Project description:Oxidation products of 1-palmitoyl-2-arachidonoyl-sn-glycerol-2-phosphatidylcholine (PAPC), referred to as OxPAPC, accumulate in atherosclerotic lesions and regulate over 1000 genes in human aortic endothelial cells (HAEC). We previously demonstrated that OxPAPC covalently binds to a number of proteins in HAEC. The goal of these studies was to gain insight into the binding mechanism and determine if binding regulates activity. In whole cells N-acetylcysteine inhibited gene regulation by OxPAPC, and blocking cell cysteines with N-ethylmaleimide strongly inhibited OxPAPC binding. Using MS, we demonstrate that most of the binding of OxPAPC to cysteine is mediated by PEIPC. We also show that OxPAPC binds to a model protein, H-Ras, at cysteines previously shown to regulate activity in response to 15dPGJ2. This binding was observed with recombinant protein and in cells overexpressing H-Ras. 15dPGJ2 and OxPAPC increased H-Ras activity at comparable concentrations. Using microarray analysis, we demonstrate a considerable overlap of gene regulation by OxPAPC and 15dPGJ2 in HAEC, suggesting that some effects attributed to 15dPGJ2 may also be regulated by OxPAPC since OxPAPC lipids and 15dPGJ2 both accumulate in inflammatory sites. Overall, we provide evidence for the importance of OxPAPC-cysteine interactions in regulating HAEC function.

2012-02-14 | GSE35709 | GEO

Effects of intermittent and chronic hypoxia on HAEC

Project description:Intermittent hypoxia (IH) in HeLa cell culture activates proinflammatory transcription factor NFκB, whereas chronic hypoxia (CH) does not. In order to determine whether IH may be linked to vascular inflammation, we developed a novel IH cell culture system and exposed HAEC (human aortic endothelial cells) to IH or CH. Keywords: Human Artery Endothelial Cells (HAEC)

2008-12-31 | GSE10723 | GEO

Evidence for the importance of OxPAPC interaction with cysteines in regulating endothelial cell function

2012-02-14 | E-GEOD-35709 | biostudies-arrayexpress

The effects of 3OC12-HSL on global gene expression in primary human aortic endothelial cells (HAEC).

Project description:Analysis of the effect of a bacterial quorum sensing molecule on in-vitro culture of human endothelial cells at gene expression levels. Objective: Chronic infection has long been postulated as a stimulus for atherogenesis. Pseudomonas aeruginosa infection has been associated with increased atherosclerosis in rats, and the bacteria produce a quorum sensing molecule 3-oxo-dodecynoyl-homoserine lactone (3OC12-HSL) that is critical for colonization and virulence. Paraoxonase 2 (PON2) hydrolyzes 3OC12-HSL and also protects against the effects of oxidized phospholipids thought to contribute to atherosclerosis. We now report the response of human aortic endothelial cells (HAEC) to 3OC12-HSL and oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (Ox-PAPC) in relation to PON2 expression. Methods and Results: Using expression profiling and network modeling, we identified the unfolded protein response (UPR), cell cycle genes, and the MAPK signaling pathway to be heavily involved in the HAEC response to 3OC12-HSL. The network also showed striking similarities to a network created based on HAEC response to Ox-PAPC, a major component of minimally-modified LDL. HAEC in which PON2 was silenced by siRNA showed increased pro-inflammatory and UPR responses when treated with 3OC12-HSL or Ox-PAPC. Conclusion: 3OC12-HSL and Ox-PAPC influence similar inflammatory pathways. Quorum sensing molecules such as 3OC12-HSL contribute to the pro-atherogenic effects of chronic infection and the anti-atherogenic effects of PON2 include destruction of quorum sensing molecules. 4 HAEC lines from different donors were treated with 3-O-C12 HSL or control (medium).

2010-12-30 | E-GEOD-26295 | biostudies-arrayexpress

The effects of 3OC12-HSL on global gene expression in primary human aortic endothelial cells (HAEC).

2010-12-30 | GSE26295 | GEO

Mock and stably infected rKSHV.219 cells (LEC, BEC, HUVEC, and HAEC) [Expt2]

Project description:Expression profiling of stably infected primary endothelial cells using a custom tiling microarray. LEC, BEC, HUVEC, and HAEC were infected with rKSHV.219 and selected with puromycin for 2 weeks. Mock infected LEC, BEC, HUVEC, and HAEC served as controls for their infected counterparts. All cells were harvested after 2 weeks of stable infection.

2013-03-29 | GSE45591 | GEO

Signatures of IL1b, IL6 and TNFa in immune cell-types

Project description:A complete profiling of inflammatory cytokines (IL1b, IL6 and TNFa) in 14 immune cell-types

2025-01-14 | GSE239946 | GEO