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Keloid tissue: active keloid lesion vs. adjacent normal skin tissue

ABSTRACT: Transcriptional profiling of active keloid lesion and adjacent control normal skin tissue from human. Each control and lesion sample was derived from a matched individual.

ORGANISM(S): Homo sapiens

PROVIDER: GSE90051 | GEO | 2017/07/01

SECONDARY ACCESSION(S): PRJNA354398

REPOSITORIES: GEO

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Keratinocyte and fibroblast gene expression in skin and keloid scar tissue

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RNA sequencing of keratinocyte gene expression in keloid and normal skin tissue

Project description:RNA sequencing has been performed to investigate the transcriptomic profile of keratinocyte derived from keloid and normal skin tissue. Keratinocytes of low passage (p2-4) were cultured onto T25 flasks until full confluence was reached. Keratinocytes were then harvested by trypsinization using 0.05% trypsin-EDTA and washed in Phosphate Buffered Saline (PBS). RNA was extracted using the RNeasy Mini kit protocol. Samples were then sent to the Australian Genome Research Facility (AGRF) where 1μg of RNA was submitted for next-generation sequencing. Once raw data returned, bioinformatics analyses were conducted. Through RNA-seq and bioinformatics analysis, 252 differentially expressed genes were identified in keloid keratinocytes compared to normal skin keratinocytes. Further gene ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) analyses revealed that changes in tight junctions and dysregulated response to viral infection were associated with keloid epithelia and may be linked to the disease. The results of the transcriptome analysis intimate that keloid derived keratinocytes exhibit an abnormal gene expression profile and the abnormalities in keloid keratinocytes may have an important role in keloid pathogenesis.

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Homo sapiens

Project description:Keloid tissue: active keloid lesion vs. adjacent normal skin tissue

| PRJNA354398 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of keloid and normal skin

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