Transcriptomics,Genomics

Dataset Information

29

Comparison of miRNA expression profile between arterial plasma and venous plasma


ABSTRACT: Circulating microRNAs (miRNAs) presented in venous plasma have recently been demonstrated as powerful biomarkers for the diagnosis and prognostic prediction of complex diseases like cancer. Nevertheless, those presented in arterial plasma have been ignored based on the assumption that the miRNA profiles in arterial and venous plasma would be identical. Here, we disputed this intuitive assumption by comparing arterial and venous plasma miRNA expression profiles from male rats using microarray technique. Though the microRNA profiles were largely similar, a considerable number of miRNAs showed significant differential expression, including 10 arterial highly expressed miRNAs and 14 venous highly expressed miRNAs. The differentially expressed miRNAs were validated by qRT-PCR. We performed computational analysis of the function enrichment and disease association of these miRNAs and their targets. Our analysis also suggested significant correlations between plasma miRNA expression and tissue miRNA expression. Four arterial highly expressed miRNAs showed enriched expression in specific tissues and thus could serve as novel biomarker candidates. Overall design: Three healthy male rats were prepared. For each rat, one arterial plasma sample and one venous plasma sample were extracted. MiRNA expression profiles in three matched pairs of arterial/venous plasma samples were measured by using microarray.

INSTRUMENT(S): Agilent-070154 Rat_miRNA_V21.0_Microarray 070153 [Probe name version]

SUBMITTER: Qinghua Cui  

PROVIDER: GSE90098 | GEO | 2016-11-22

SECONDARY ACCESSION(S): PRJNA354413

REPOSITORIES: GEO

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Publications

Transcriptome analysis reveals non-identical microRNA profiles between arterial and venous plasma.

Xu Wenjing W   Zhou Yuan Y   Xu Guoheng G   Geng Bin B   Cui Qinghua Q  

Oncotarget 20170401 17


Circulating microRNAs presented in venous plasma have been demonstrated as powerful biomarkers for the complex diseases like cancer. Nevertheless, those presented in arterial plasma remained largely unexplored. Here, using microarray technique, we compared microRNA expression profiles of the matched arterial and venous plasma samples from the same male rats. Though the microRNA profiles were largely similar, we identified 24 differentially expressed microRNAs, including 10 arterial highly expres  ...[more]

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