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DNase-seq from Daoy (ENCSR346IHH)

ABSTRACT: DNase-seq on cell line Daoy (polygonal cell from 4 year old male human cerebellum, desmoplastic cerebellar medulloblastoma) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

ORGANISM(S): Homo sapiens

PROVIDER: GSE90323 | GEO | 2016/12/07

REPOSITORIES: GEO

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RAMPAGE from Daoy (ENCSR700NMZ)

Project description:The libraries contained in this experiment come from independent growths of cell line Daoy isolated from the cerebellar region of a 4 year old caucasian male with desmoplastic cerebellar medulloblastoma. They are stranded PE101 Illumina Hi-Seq RAMPAGE libraries from rRNA-depleted Total RNA > 200 nucleotides in size. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

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Daoy gene expression

Project description:Using measurements of absolute protein and mRNA concentrations in cellular lysate from a Daoy medulloblastoma cell line, we quantitatively evaluate the influence of absolute transcript levels and a large number of sequence-encoded regulatory elements on steady-state human protein expression levels.

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Daoy gene expression

2010-04-22 | E-GEOD-20492 | biostudies-arrayexpress

Culturing of Daoy cells in astrocyte conditioned media

Project description:Astrocytes are important cells within the medulloblastoma microenvironment. Therefore, we assessed how astrocyte secreted factors may alter Daoy (human MB cell line; ATCC HTB-186) cell gene expression.

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LC-MS/MS analysis of Daoy and UW228 medulloblastoma cell lines

Project description:The goal of this study was to profile the total proteome and transcriptome of the established medulloblastoma cell lines, Daoy and UW228, using label-free nano-LC-MS/MS-based quantitative proteomics and high-throughput RNA sequencing (RNA-Seq), coupled with pathway analysis to identify differentially expressed genes, proteins and signaling pathways with potential as prognostic markers. A total of 14250 and 12870 transcripts were detected for Daoy and UW228, respectively. Proteomic profiling identified 2630 and 1235 proteins in Daoy and UW228, representing 18% and 10% of detected transcripts, respectively. Interestingly, Daoy proteome included >50% unique proteins, while almost 90% of proteins expressed by UW228 were commonly expressed in Daoy. Differential expression of a number of adhesion, cytoskeletal and signaling molecules were observed between the two cell lines. Upregulation of a number of proteins and enrichment of key signaling pathways, including WNT, Sonic hedgehog (SHH) and integrin signaling pathways, were uniquely observed in MB cell lines, in particular in Daoy.

2017-10-27 | PXD002659 | Pride

total RNA-seq from Daoy (ENCSR254JJM)

Project description:The libraries contained in this experiment come from the whole cell fraction of independent growths of cell line Daoy. They are stranded PE101 Illumina Hi-Seq RNA-Seq libraries from rRNA-depleted Total RNA > 200 nucleotides in size. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2016-03-18 | GSE78636 | GEO

DNase-seq from A673 (ENCSR346JWH)

Project description:DNase-seq on cell line A673 (polygonal cell from 15 year old female human muscle, Ewing's sarcoma) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

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RNA sequencing analysis of stable medulloblastoma DAOY, D425, and CHLA01 cells expressing vector only (V/O) or miR-211

Project description:To investigate the role of miR-211 in medulloblastoma, next-generation sequencing analysis of RNA extracted from DAOY, D425, and CHLA01 cells expressing vector only (V/O) or miR-211 were performed.

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Growth factor-induced alterations of plasma membrane-associated proteome of DAOY cell

Project description:In this study we investigated alterations in the plasma membrane (PM)-associated proteome of DAOY medulloblastoma cells. We used a surface biotinylation approach to label PM-associated proteins. We enriched for biotinylated proteins by streptavidin-pull-down and determined the composition by mass-spectrometry. We compared CRISPR/CAS9 control cells and CRISPR/CAS9 MAP4K4 (MAP4K4 depletion) cells under starvation and after 30 min stimulation with 20 ng/ml HGF. We performed two group analyses of all conditions.

2022-03-14 | PXD030597 | Pride

circRNA and mRNA transcriptome of Human Daoy and HEPM cells in the context of Hedgehog signaling activation

Project description:Circular RNAs have largely emerged as novel regulators of human biology, including brain function and cancer development. On the other hand, the Hedgehog pathway has established roles in regulating biological processes, including tumorigenesis. Here, the circular RNA transcriptome, in the context of Hedgehog signaling activation of medulloblastoma Daoy and human embryonic palatal mesenchyme HEPM cells, was determined.

2021-10-13 | GSE183194 | GEO

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