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Expression data of HUVEC cells after TNF-alpha treatment

ABSTRACT: Tumor necrosis factor a (TNF-aplha) is a potent cytokine that orchestrates the inflammatory response by sequentially activating the expression of many genes in cultured human umbilical vein cells (HUVECs). We apply samples collected every 7.5 min to arrays and monitor the growth of several long transcripts. Keywords: time course

ORGANISM(S): Homo sapiens

PROVIDER: GSE9036 | GEO | 2007/09/19

SECONDARY ACCESSION(S): PRJNA102539

REPOSITORIES: GEO

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Expression data of HUVEC cells after TNF-alpha treatment

Project description:Tumor necrosis factor a (TNF-aplha) is a potent cytokine that orchestrates the inflammatory response by sequentially activating the expression of many genes in cultured human umbilical vein cells (HUVECs). We apply samples collected every 7.5 min to arrays and monitor the growth of several long transcripts. Keywords: time course Before stimulation, HUVECs were serum-starved in EBM-2 (Clonetics) containing 0.5% FBS for 18 h, and then treated with 10 ng/ml TNF (Peprotec) and samples collected every 7.5 min for 3 h.

2007-09-19 | E-GEOD-9036 | biostudies-arrayexpress

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RNA sequencing of HECTD3 knockdown during TNFalpha stimulation in primary HUVEC cells

Project description:We isolated and validated primary HUVECs from the neonatal umbilical cord vein, and performed genome-wide expression analysis to profile differentially expressed genes in control and HECTD3-KD HUVECs treated with or without TNFα for 2 hours. Compared with control HUVECs, 59 genes showed lower expression in HECTD3-KD HUVECs in response to TNFα. Most of them are NF-κB target genes, including adhesion molecules SELE (E-selectin), ICAM-1, and VCAM-1.

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Real-time quantitative PCR analysis of Human Umbilical Vein Endothelial Cells (HUVEC)

Project description:In order to identify new markers of vascular cell senescence with potential in vivo implications, primary cultured Human Umbilical Vein Endothelial Cells (HUVECs), were analysed for microRNA (miR) expression. QRT-PCR microRNA expression profiling in 3 senescent (XIII passage) vs. 3 young HUVECs (II passage).

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Effects of AEBP1 knockdown on gene expression profiles in HUVEC

Project description:We identified adipocyte enhancer binding protein 1 (AEBP1) as a novel tumor endothelial marker in colorectal cancer (CRC). To identify target genes of AEBP1 in endothelial cells, we knocked down AEBP1 in human umbilical vein endothelial cells (HUVECs). We found that genes associated with angiogenesis including aquaporin 1 (AQP1) and periostin (POSTN) are downregulated by AEBP1 knockdown in HUVECs, suggesting that AEBP1 may promote tumor angiogenesis through regulating these genes.

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Expression data from HUVEC adenovirally overexpressing MEF2C

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2013-06-01 | GSE46279 | GEO

Expression data from HUVEC adenovirally overexpressing MEF2C

2013-06-01 | E-GEOD-46279 | biostudies-arrayexpress

Interaction effects of knockdown of PML and TNFalpha treatment in HUVEC cells

Project description:To study the interaction effects between promyelocytic leukemia (PML) gene knockdown and tumor necrosis factor-alpha (TNFalpha) signaling in human umbilical vein endothelial cells (HUVECs), we transfected control or two independent PML siRNAs into HUVEC cells without or with 20 ng/mL TNFalpha treatment for 20 h. The total RNA was extracted for gene expression microarray analyses.

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