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Identifying ASCL1-mediated chromatin changes in primary GBM stem cell cultures [ATAC-seq]

ABSTRACT: ASCL1 mediates neuronal differentiation of GBM stem cell (GSC) cultures. We sought to identify chromatin changes upon induced ASCL1 expression in primary human GSC cultures. In this dataset, we include ATAC-seq data obtained from GSC cultures harbouring a CRISPR-deletion of ASCL1. We assessed differential ASCL1 binding between control and GSC cultures induced to overexpress ASCL1 after 14 days of doxycycline treatment.

ORGANISM(S): Homo sapiens

PROVIDER: GSE90547 | GEO | 2017/07/13

SECONDARY ACCESSION(S): PRJNA354999

REPOSITORIES: GEO

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Identifying ASCL1 target genes in primary GBM stem cell cultures [ChIP-seq]

Project description:ASCL1 mediates neuronal differentiation of GBM stem cell (GSC) cultures. We sought to identify genomic targets of ASCL1 in primary human GSC cultures. In this dataset, we include ChIP-seq data obtained from GSC cultures harbouring a CRISPR-deletion of ASCL1. We assessed differential ASCL1 binding between control and GSC cultures induced to overexpress ASCL1 after 18 hours of doxycycline treatment.

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