Transcriptomics,Genomics

Dataset Information

18

Short-term Activin A and BMP4 stimulation experiments with human ES cells


ABSTRACT: This experiment was carried out to identify the short-term effects of Activin A and BMP4 stimulation on gene expression in human embryonic stem cells grown in a chemically defined medium. Keywords: Growth factor stimulation experiment Overall design: hES cells were adapted to chemically defined medium, replated, and, three days after passaging, stimulated with either recombinant Activin A or BMP4. Data are from independent experiments (distinct non-stimulated controls for Activin A and BMP4 experiments - two biological replicates each).

INSTRUMENT(S): Sentrix HumanRef-8 Expression BeadChip

SUBMITTER: Boris Greber  

PROVIDER: GSE9060 | GEO | 2008-03-12

SECONDARY ACCESSION(S): PRJNA105319

REPOSITORIES: GEO

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Publications

Control of early fate decisions in human ES cells by distinct states of TGFbeta pathway activity.

Greber Boris B   Lehrach Hans H   Adjaye James J  

Stem cells and development 20081201 6


The mechanisms controlling self-renewal versus lineage commitment in human embryonic stem (hES) cells are not well understood. Nonetheless, current knowledge suggests a crucial role for TGFbeta signaling in controlling these early fate decisions. We have investigated the effects of TGFbeta pathway activation and inhibition on gene expression in hES cells. Our data reveal that SMAD 2/3 signaling directly supports NANOG expression, while SMAD 1/5/8 activation moderately represses SOX2. In addition  ...[more]

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