Transcriptomics,Genomics

Dataset Information

22

Comparative expression analysis of the Arabidopsis karrikin (KAR)-receptor mutant, kai2-2, and wild type (WT) plants (Col-0) under dehydration conditions


ABSTRACT: To understand the role of KAR-signaling components in water stress response, we have carried out comparative expression analysis of the KAR-receptor kai2-2 mutant and WT plants under dehydration and well-watered (control) conditions. Aligent’s whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used. Overall design: Two-week-old WT and kai2-2 mutant plants were transferred from GM plates to soil and grown for 10 additional day. The aerial parts of 24-d-old plants were detached and exposed to dehydration on KimTowel papers for 0 (well-watered, control), 2 and 4 h. All rosette leaves of independent 24-d-old plants were collected. Total RNA was prepared and used for the microarray hybridization. Three independent biological replicates were used for each plant sample.

INSTRUMENT(S): Agilent-021169 Arabidopsis 4 Oligo Microarray (V4) (Probe Name version)

SUBMITTER: Ha Van Chien  

PROVIDER: GSE90622 | GEO | 2017-10-15

SECONDARY ACCESSION(S): PRJNA355200

REPOSITORIES: GEO

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