Transcriptomics,Genomics

Dataset Information

28

Activin A-driven differentiation timecourse with human embryonic stem cells


ABSTRACT: Activin A is known to induce endodermal differentiation in human embryonic stem cells under low-serum or certain defined media conditions. To gain insights into global gene expression changes during Activin A-induced differentiation, gene expression was monitored by means of microarrays in short intervals until 4 days of treatment. Keywords: Differentiation timecourse experiment Overall design: hES cells were adapted to N2B27-based chemically defined medium, replated and continuously treated with Activin A in the absense of FGF2. Cells from replicate cultures were harvested 0, 12, 24, 36, 48, 72, and 96 hours after the inititation of Activin A stimulation. Each timepoint was represented by two biological replicates.

INSTRUMENT(S): Sentrix HumanRef-8 Expression BeadChip

SUBMITTER: Boris Greber  

PROVIDER: GSE9063 | GEO | 2008-03-12

SECONDARY ACCESSION(S): PRJNA105323

REPOSITORIES: GEO

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Publications

Control of early fate decisions in human ES cells by distinct states of TGFbeta pathway activity.

Greber Boris B   Lehrach Hans H   Adjaye James J  

Stem cells and development 20081201 6


The mechanisms controlling self-renewal versus lineage commitment in human embryonic stem (hES) cells are not well understood. Nonetheless, current knowledge suggests a crucial role for TGFbeta signaling in controlling these early fate decisions. We have investigated the effects of TGFbeta pathway activation and inhibition on gene expression in hES cells. Our data reveal that SMAD 2/3 signaling directly supports NANOG expression, while SMAD 1/5/8 activation moderately represses SOX2. In addition  ...[more]

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