ChIP-Seq of constitutively expressed Ndt80-Myc in K. lactis

ABSTRACT: The purpose of this experiment was to identify the genes bound by Ndt80 in K. lactis when constitutively expressed. Ndt80 was tagged with c-myc, the native Ndt80 promoter was replaced with the K. lactis Gal1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown overnight in SRaffinose until log-phase, then in SRaffinose + 1.7% Galactose for 5 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000.

ORGANISM(S): Kluyveromyces lactis

PROVIDER: GSE90662 | GEO | 2017/05/01

SECONDARY ACCESSION(S): PRJNA355301

REPOSITORIES: GEO