Genomics

Dataset Information

0

Molecular characterisation of High Beta Carotene (HBC) Mutant


ABSTRACT: The High-β-Carotene (HBC) mutant identified from EMS mutagenized population of cultivar Arka Vikas and the biochemical studies revealed that mutant fruits contain four times higher level of β-Carotene in comparison to Wild type (cv. Arka Vikas). We have developed whole genome microarray expression profiling as a discovery platform to identify differentially expressed genes in fruits containing High-β-Carotene mutant in comparison to Wild type (cv. Arka Vikas). Tomato fruit tissue samples from different stages of fruit ripening like Mature green, Breaker, Turning and Ripening stages of High-β-Carotene (HBC) mutant and Wild type (cv. Arka VIkas) were used for microarray gene expression analysis. Carotenoid pathway analysis of both mutant and wild type reveals that the high expression of chromoplast specific lycopene- β-cyclase gene in the HBC mutant, which is involved in the conversion of lycopene to β-carotene, but in wild type the expression of this gene, was low. Four genes (PSY, PDS, CRTISO, CYCB) of the carotenoid pathway was quantified in the same RNA samples by real-time PCR, confirming that the variation of the gene expression in HBC mutant.

ORGANISM(S): Solanum lycopersicum

PROVIDER: GSE90964 | GEO | 2017/12/01

SECONDARY ACCESSION(S): PRJNA356700

REPOSITORIES: GEO

Similar Datasets

2018-12-01 | GSE91045 | GEO
2020-01-01 | GSE108415 | GEO
2018-12-01 | GSE92645 | GEO
2012-12-12 | GSE33435 | GEO
2016-09-14 | GSE86866 | GEO
| E-GEOD-33435 | biostudies-arrayexpress
| PRJNA356700 | ENA
| E-GEOD-49289 | biostudies-arrayexpress
2014-06-23 | GSE49289 | GEO
2017-06-22 | GSE100319 | GEO