Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Global gene expression profiling and senescence biomarker analysis of hESC exposed to H2O2 induced non-cytotoxic oxidative stress

ABSTRACT: Human embryonic stem cells (hESCs) potentially offer new routes to study, on the basis of the Developmental Origins of Health and Disease (DOHaD) concept, how the maternal environment during pregnancy influences the offspring health and can predispose to chronic disease in later life. Reactive Oxygen Species (ROS), antioxidant defences, and cellular redox status state play an important role in the regulation of gene expression and are involved in diabetes and metabolic syndromes as in aging. We designed an in vitro cell model of oxidative stress by exposing hESCs to hydrogen peroxide (H2O2) for 72h. To resemble the period of preimplantation embryonic development, we have analysed the global gene expression profiles of hESCs (HUES3) exposed to non-cytotoxic H2O2 concentrations (see main text), using Illumina HT-12 v4 expression beadarray.

ORGANISM(S): Homo sapiens

PROVIDER: GSE90999 | GEO | 2017/08/30

SECONDARY ACCESSION(S): PRJNA356617

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNA sequencing of hydrogen peroxide (H2O2)-treated melanocytes

Project description:Purpose: Determine the mechanism of H2O2-induced signaling in melanocytes. Method: Primary human epidermal melanocytes were treated with H2O2 (200 μM) and incubated for 24 h. Total RNA (500 ng) from melanocytes were extracted and subjected to library synthesis. Results: H2O2-treated melanocytes exhibited upregulation of cell death and type 1 interferon-related genes. Conclusion: H2O2-induced melanocyte signaling was well evaluated using RNA sequencing.

2023-07-28 | GSE216447 | GEO

Effect of ROS (H2O2) on undifferentiated NSC

Project description:Release of reactive oxygen species, such as H2O2, has been observed during inflammatory processes but is also a physiological side product of metabolism. We have observed that differentiation of neural progenitor cells (NPC) is affected by H2O2 exposure. We here assess the transcriptional drivers of the phenotypical changes NPCs undergo during differentiation in response to H2O2. Here we publish only the untreated controls.

2023-05-11 | GSE232232 | GEO

Atf1-mediated active gene response to H2O2 challenge.

Project description:Here we present the study on ChIP-chip mapping of the genomic binding sites for Sty1, Atf1, and the Atf1's binding partner Pcr1; the genome-wide transcriptional profiling of the atf1 and pcr1 strains in response to H2O2; and the phenotypic assessment of ~90 Atf1/Pcr1-bound or unbound genes for growth fitness under H2O2 conditions. ChIP-chip analysis shows that Atf1 and Pcr1 binding sites are overlapped in the genome and constitutively present before H2O2 stress. On the other hand, Sty1 recruitment primarily occurs at the Atf1/Pcr1 binding sites and is induced by H2O2. We found that Atf1/Pcr1 is clearly responsible for the high-level transcriptional response to H2O2. Furthermore, phenotypic assessment indicates that among the H2O2-induced genes, Atf1/Pcr1-bound genes exhibit a higher likelihood of functional requirement for growth fitness under the stress condition than the Atf1/Pcr1-unbound genes do. Notably, we found that the Atf1/Pcr1-bound genes regardless of their responsiveness to H2O2 show a high probability of requirement for growth fitness. .

2010-07-19 | GSE13053 | GEO

Halobacterium NRC-1, H2O2 Stress Response

Project description:50mM H2O2 was added to mid-log phase Halobacterium NRC-1 cultures. After constant stress of H2O2 for 30 minutes, cultures were spun down and pellets were resuspended in same volume of GN101 media. Samples for RNA preparation were collected during recovery time points at 0, 10, 20, 30, 40, 60 and 120 minutes. Keywords: stress response

2008-01-03 | GSE7610 | GEO

RNA-seq of definitive endoderm differentiation of human embryonic stem cells in early G1 phase of the cell cycle

Project description:The experiment was designed to investigate transcriptional dynamics during definitive endoderm differentiation of human embryonic stem cells (hESCs) in early G1 phase of the cell cycle. For this, the FUCCI system was used to sort hESCs in Early G1 (EG1). Samples were taken at 0h (hESCs), 12h, 24h, 36h, 48h, 60h and 72h in triplicates. Total RNA was extracted using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich) and residual genomic DNA was removed using the On-Column DNase I Digestion Set (Sigma-Aldrich) following manufacturer’s instructions. The libraries were generated at a library fragment size between 100 bp to 1 kb with Stranded RNAseq Standard with Oligo dT pulldown. All samples were amplified with a standard 10 PCR cycle before sequencing. The samples were multiplexed and analysed by Illumina Hiseq V4 with a paired end read-length PE75. RNA-seq data analysis was performed following recommendations described in Conesa et al. (DOI https://doi.org/10.1186/s13059-016-0881-8).

2020-09-07 | E-MTAB-9194 | biostudies-arrayexpress

H2O2 Treatment of Human PBMC

Project description:To mimic the effect of ROS in contributing to ageing process in immune cells of PBMC. The PBMC cells were harvested from old human subjects (>50) and induced with H2O2 during 3 hour and 6 hour. The CEL file were normalized using RMA methods. We used microarray to explore the gene expression profiling before and after ROS. Gene related to cell cycle, apoptosis, metabolism and signal transduction were differentially expressed after inducing with H2O2 in compared to baseline.

2010-06-20 | GSE22463 | GEO

H2O2 Treatment of Human PBMC

2010-07-08 | E-GEOD-22463 | biostudies-arrayexpress

Halobacterium NRC-1, H2O2 Stress Response

2010-05-27 | E-GEOD-7610 | biostudies-arrayexpress

Bj H2O2 shock vs. non-treatment

Project description:Genome-wide transcriptional profiling of cells subjected to a H2O2 shock treatment (10 mM for 10 minutes).

2011-07-30 | GSE26961 | GEO

H2O2-treated and untreated HCT116 cells

Project description:H2O2-treated and untreated HCT116 cells

| PRJNA305862 | ENA