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Cystathionine-β-Synthase Promotes Colon Carcinogenesis

ABSTRACT: Purpose: The goal of this study is to investigate the role of CBS enzyme in colorectal carcinogenesis Methods: RNA-Seq transcriptome analysis of CBS-overexpression in NCM356 cels compared to control vector cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE91000 | GEO | 2017/12/07

SECONDARY ACCESSION(S): PRJNA356616

REPOSITORIES: GEO

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Cystathionine beta synthase (CBS) Over expression vs PLenti6 Expression Vector Human Bronchial Epithelial Cells (HBEC)

Project description:Cystathionine Beta Synthase (CBS), an enzyme that utilizes a methionine precursor to generate glutathione (GSSH), has been implicated in the mitigation of ROS production in COPD lung. Additionally, post-translational modification analysis on mouse and human cells treated with cigarette smoke showed the unique presence of mono- and di-methylated histone H3 lysine 27 that was not seen in controls, suggesting that cigarette smoke could lead to loss of Polycomb Repressive Complex 2 (PRC2) activity. To elucidate CBS as a potential upstream target for PRC2 inhibition, we overexpressed CBS in Human Bronchial Epithelial Cells (HBECs), grew them as 2D cultures, then conducted bulk RNA seq analysis.

2022-06-30 | GSE183858 | GEO

Endogenous Elevation of Homocysteine Induces Retinal Neuron Death in the Cystathionine-b-Synthase Mutant Mice

Project description:The effects of endogenous elevation of homocysteine on retinal gene expression were screened by microarray analysis, which was performed in triplicate (i.e., three different paired RNA samples) with RNA isolated from neural retinas of cbs+/- HM at 5-weeks after a methionine diet and age-matched wildtype mice. The microarray chips used in this study permitted the screening of 36,212 genes. Of particular interest were those genes in cbs+/- HM mice whose expression levels changed by at least a factor of 2 (increased or decreased expression) compared with the wild-type in each of the replicate experiments. Using these selection criteria, we identified 1219 genes. They were categorized into six functional groups: (1) proapoptosis, (2) antiapoptosis, (3) cell cycle, (4) antioxidant, (5) calcium signaling, and (6) axon growth/guidance, with the remaining genes with marked changes in expression placed in a miscellaneous group. After categorization, relevant genes were further narrowed by SAM analysis; q < 0.06 was considered significant. Microarrays were performed in triplicate as biological repeats and reverse fluoro (i.e., three different paired RNA samples) with RNA isolated from neural retinas of cbs+/- HM at 5-weeks after a methionine diet and age-matched wildtype mice after a methionine diet.

2014-04-04 | E-GEOD-56458 | biostudies-arrayexpress

Endogenous Elevation of Homocysteine Induces Retinal Neuron Death in the Cystathionine-b-Synthase Mutant Mice

2014-04-04 | GSE56458 | GEO

Effect of group III phospholipase A2 deletion in azoxymethane-induced colon carcinogenesis modeled colon

Project description:Lipid mediators play pivotal roles in colorectal cancer and colitis, but only a limited member of the phospholipase A2 (PLA2) subtypes, which lie upstream of various lipid mediators, have been implicated in the positive or negative regulation of these diseases. Clinical and biochemical evidence suggests that secreted PLA2 group III (sPLA2-III) is associated with colorectal cancer, although its precise role remains obscure. Here we have found that sPLA2-III-null (Pla2g3-/-) mice are highly resistant to colon carcinogenesis. Furthermore, Pla2g3-/- mice are less susceptible to dextran sulfateinduced colitis, implying that the amelioration of colonic inflammation by sPLA2-III ablation may underlie the protective effect against colon cancer. Lipidomics analysis of the colon revealed significant reduction of pro-inflammatory/pro-tumorigenic lysophosholipids as well as unusual elevation of colon-protective fatty acids and their oxygenated metabolites in Pla2g3-/- mice. Overall, our results establish a role of sPLA2-III in the promotion of colorectal inflammation and cancer, expand our understanding of the divergent roles of multiple PLA2 enzymes in the gastrointestinal tract, and point to sPLA2-III as a novel druggable target for colorectal diseases.

2017-11-01 | GSE102389 | GEO

Upregulation of Cystathionine-?-Synthase in Colonic Epithelia Reprograms Metabolism and Promotes Carcinogenesis.

Project description: Not available

| S-EPMC5668191 | biostudies-literature

Lipid engulfed macrophages interfere with protective T cell immunity and favor tumor progression in human colon carcinogenesis

Project description:Obesity and altered lipid metabolism is associated with colorectal cancer (CRC). In this context, the presence of colonic lipid-engulfed macrophages (foam cells, FC) may be involved in hindering protective T cell immunity, thus favoring gut carcinogenesis.

2023-03-19 | GSE227206 | GEO

PRSS8 Suppresses Colorectal Carcinogenesis and Metastasis.

Project description:PRSS8 Suppresses Colorectal Carcinogenesis and Metastasis.

| PRJNA475479 | ENA

ZFP90 promotes colorectal carcinogenesis and stemness

Project description:ZFP90 promotes colorectal carcinogenesis and stemness

| PRJNA497880 | ENA

METTL3 promotes carcinogenesis in colorectal cancer

Project description:METTL3 promotes carcinogenesis in colorectal cancer

| PRJNA533611 | ENA

Fusobacterium nucleatum promotes colorectal carcinogenesis via an m6A dependent pathway

Project description:Previous reports have indicated that RNA m6A modification plays an important role in colorectal carcinogenesis. We hypothesized that F. nucleatum promoted colorectal carcinogenesis dependent on RNA m6A alterations. To test this hypothesis, we conducted a RNA-seq analysis in HT29 cells cocultured with F. nucleatum and medium control.

2022-12-01 | GSE191257 | GEO

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