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Copper(II) Sulfate treatment with 10 mM for 2 h

ABSTRACT: The effect of the heavy metal copper on the expression of a wide spectrum of genes was analyzed by using a DNA microarray. Gene expression profile of baker’s yeast Saccharomyces cerevisiae grown in a media containing sub-lethal concentration of cupric sulfate was compared with those of yeast grown in a normal media. Among about 6200 ORFs of yeast, 143 ORFs were induced more than two-fold to resist against copper toxicity after exposure to copper. As was expected, FRE1, FRE7, and CTR1, genes controlled by Mac1p, were strongly down-regulated. Copper metallothionein CUP1-1 and CUP1-2 were induced more than 20-fold. Some genes related to sulfur metabolism and oxidative stress response were also up-regulated. This DNA microarray technology indicated that plasma membrane, cell wall, protein, and DNA, were molecular targets of copper toxicity. Keywords: stress response

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE9231 | GEO | 2007/11/01

SECONDARY ACCESSION(S): PRJNA102833

REPOSITORIES: GEO

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Over-expression of CTR1 delta-300 alters element and transcription profiles in yeast

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