Transcriptomics,Genomics

Dataset Information

165

Transcriptome assay of maternal mRNA degradation in ERK-deleted oocytes


ABSTRACT: We analyzed the functions of ERK in maternal mRNA degradation in mouse oocytes. By comparing the degradation of transcripts in WT oocytes and KO oocytes, we are able to know the defects in maternal mRNA clearance in ERK-deleted oocytes, and identified the ERK target genes in oocyte maturation. Overall design: a WT GV oocyte, a WT MII oocyte, a Erk-/- GV oocyte and a Erk-/- MII oocyte are performed RNA sequencing.

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: yujiao dang  

PROVIDER: GSE92317 | GEO | 2016-12-16

SECONDARY ACCESSION(S): PRJNA357208

REPOSITORIES: GEO

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Publications

A MAPK cascade couples maternal mRNA translation and degradation to meiotic cell cycle progression in mouse oocytes.

Sha Qian-Qian QQ   Dai Xing-Xing XX   Dang Yujiao Y   Tang Fuchou F   Liu Junping J   Zhang Yin-Li YL   Fan Heng-Yu HY  

Development (Cambridge, England) 20161219 3


Mammalian oocyte maturation depends on the translational activation of stored maternal mRNAs upon meiotic resumption. Cytoplasmic polyadenylation element binding protein 1 (CPEB1) is a key oocyte factor that regulates maternal mRNA translation. However, the signal that triggers CPEB1 activation at the onset of mammalian oocyte maturation is not known. We provide evidence that a mitogen-activated protein kinase (MAPK) cascade couples maternal mRNA translation to meiotic cell cycle progression in  ...[more]

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