Transcriptomics,Genomics

Dataset Information

39

Expression data for human colon cancer cell line HCT116 transfected with siRNA targeting YAP1.


ABSTRACT: Yes-associated protein 1 (YAP1) is an effector of Hippo pathway, which is critical for regulating organ size, cell proliferation and tumor growth in mammals. YAP1 is known to be involved in tumorigenesis in several tissues, yet its role in colorectal cancer(CRC) is not established. To investigate the effect of YAP1 in CRC, we used microarrays to compared human colon cancer cell line HCT116 transfected with a control non-targeting siRNA to cells and transfected with siRNA targeting YAP1. Overall design: Human colon cancer cell line HCT116 was transfected with a control non-targeting siRNA to cells or transfected with siRNA targeting YAP1 for 36 hours in DMEM medium (with 2% serum). Total RNA were extracted and detected by Affymetrix Human Genome U133 Plus 2.0 Array. 3 independent biological replicates were plated, transfected in parallel for each control and siRNA.

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

SUBMITTER: Jian Ma  

PROVIDER: GSE92335 | GEO | 2018-01-01

SECONDARY ACCESSION(S): PRJNA357257

REPOSITORIES: GEO

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Publications

MiR-590-5p, a density-sensitive microRNA, inhibits tumorigenesis by targeting YAP1 in colorectal cancer.

Ou Chunlin C   Sun Zhenqiang Z   Li Xiayu X   Li Xiaoling X   Ren Weiguo W   Qin Zailong Z   Zhang Xuemei X   Yuan Weitang W   Wang Jia J   Yu Wentao W   Zhang Shiwen S   Peng Qiu Q   Yan Qun Q   Xiong Wei W   Li Guiyuan G   Ma Jian J  

Cancer letters 20170419


YAP1, a transcription co-activator, mediates the biological functions of the Hippo pathway. YAP1 inactivation is involved in cell-cell contact inhibition. In various tumors, YAP1 is upregulated through multiple mechanisms, and it functions as an oncogene. Here, we provided evidence that YAP1 influenced multiple signaling pathways in colorectal cancer (CRC) cells. We reported that miR-590-5p directly targets YAP1 and inhibits tumorigenesis in CRC cells both in vitro and in vivo xenograft model. W  ...[more]

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