Transcriptomics,Genomics

Dataset Information

42

AAV-mediated RNAi of traumatic brain injury-induced genes in the rat hippocampus


ABSTRACT: To address the hypothesis that silencing deleterious or protective injury-induced genes in the rat hippocampus will reduce or increase the numbers of injured hippocampal neurons, alter cellular pathways essential for neuronal function and improve or worsen functional outcome after traumatic brain injury (TBI), we evaluated the effects of silencing neuronal nitric oxide synthase (nNOS) and glutathione peroxidase-1 (GPx-1) expression in the injured rat hippocampus. Overall design: Two weeks post-TBI/post-AAV treatment, hippocampal pyramidal neurons were laser captured for comparative whole genome microarray analysis.

INSTRUMENT(S): Agilent-028282 Whole Rat Genome Microarray 4x44K v3 (Probe Name version)

SUBMITTER: Michael Falduto  

PROVIDER: GSE92363 | GEO | 2018-02-16

REPOSITORIES: GEO

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Publications


Virally mediated RNA interference (RNAi) to knock down injury-induced genes could improve functional outcome after traumatic brain injury (TBI); however, little is known about the consequences of gene knockdown on downstream cell signaling pathways and how RNAi influences neurodegeneration and behavior. Here, we assessed the effects of adeno-associated virus (AAV) siRNA vectors that target two genes with opposing roles in TBI pathogenesis: the allegedly detrimental neuronal nitric oxide synthase  ...[more]

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