Transcriptomics,Genomics

Dataset Information

157

Tsc2 KO cells have a transcriptional signature found in bladder tumors with TSC1/2 deficiency and that is corrected by sirolimus and includes Lgals3 overexpression


ABSTRACT: Use RNA-seq to obtain transcriptional signature of Tsc2 KO neonatal mouse dermal fibroblasts +/- sirolimus. The resulting mTORC1 transcriptional signature is tested in the TCGA database of bladder cancers with non-silent mutations in TSC1/2. Overall design: n = 3 WT vehicle, n = 3 KO vehicle, n = 3 WT sirolimus, n = 3 KO sirolimus

INSTRUMENT(S): Illumina NextSeq 500 (Mus musculus)

SUBMITTER: Thomas Darling  

PROVIDER: GSE92589 | GEO | 2017-06-29

SECONDARY ACCESSION(S): PRJNA358080

REPOSITORIES: GEO

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Publications


Increased mTORC1 signaling from TSC1/TSC2 inactivation is found in cancer and causes tuberous sclerosis complex (TSC). The role of mesenchymal-derived cells in TSC tumorigenesis was investigated through disruption of Tsc2 in craniofacial and limb bud mesenchymal progenitors. Tsc2cKOPrrx1-cre mice had shortened lifespans and extensive hamartomas containing abnormal tortuous, dilated vessels prominent in the forelimbs. Abnormalities were blocked by the mTORC1 inhibitor sirolimus. A Tsc2/mTORC1 exp  ...[more]

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