Transcriptomics,Genomics

Dataset Information

49

Gene expression of settled and metamorphosed Orbicella faveolata during establishment of symbiosis


ABSTRACT: Corals rely on a symbiosis with dinoflagellate algae (Symbiodinium spp.) to thrive in nutrient poor tropical oceans. However, the coral-algal symbiosis can break down during bleaching events, potentially leading to coral death. While genome-wide expression studies have shown the genes associated with the breakdown of this partnership, the full conglomerate of genes responsible for the establishment and maintenance of a healthy symbiosis remains unknown. Results from previous studies suggested little transcriptomic change associated with the establishment of symbiosis. In order to elucidate the transcriptomic response of the coral host in the presence of its associated symbiont, we utilized a comparative framework. Post-metamorphic aposymbiotic coral polyps of Orbicella faveolata were compared to symbiotic coral polyps 9 days after metamorphosis and the subsequent differential gene expression between control and treatment was quantified using cDNA microarray technology. Coral polyps exhibited differential expression of genes associated with nutrient metabolism and development, providing insight into pathways turned as a result of symbiosis driving early polyp growth. Furthermore, genes associated with lysosomal fusion were also upregulated, suggesting host regulation of symbiont densities soon after infection. Overall design: RNA from 3 control and 3 infected polyp samples were hybridized in a 3-replicate pooled reference (6 total hyb's)

INSTRUMENT(S): UCM_Medina_Mfaveolata_11k_v1

SUBMITTER: Monica Medina 

PROVIDER: GSE92695 | GEO | 2016-12-22

SECONDARY ACCESSION(S): PRJNA358386

REPOSITORIES: GEO

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