Distinct mesenchymal lineages and niches promote epithelial self-renewal and myofibrogenesis in the lung

ABSTRACT: Using flourescence activated cell sorting, we gated on cells negative for CD45, CD326 and CD31, from single cell suspension generated from Adult mice. We then isolated subsets of YFP-, GFP- or TdTomato expressing cells from the indicated reporter mice. tdTomato indicates Wnt-responsive cells, GFP reports Pdgfr-alpha expression and YFP- was used to isolate lineaged Wnt2-positive cells. Additionally we included cells that were negative for the aforementioned reporters. We performed RNAseq on biological duplicates for each subset.

ORGANISM(S): Mus musculus

PROVIDER: GSE92699 | GEO | 2017/09/11

SECONDARY ACCESSION(S): PRJNA358398

REPOSITORIES: GEO