Transcriptomics,Genomics

Dataset Information

87

Transcriptome analysis of Agrobacterium sp. ATCC 31749 wild strain and ΔfnrN based on RNA-seq


ABSTRACT: Purpose:first,we want to find the genes revelant to curdlan synthesis and oxygen regulation, second, we want to research the function of fnrN gene in Agrobacterium sp. ATCC 31749. Method: samples of cell growth phase, curdlan-producing phase (normoxia) and curdlan-producing phase (micro-oxygen treated) in both Agrobacterium sp. ATCC 31749 wild strain and ΔfnrN strain were collectecd to extract mRNA. Each sample was treated in duplicate. The softwares we used include fastqc, trimmomatic, TopHat2 and Cufflinks. Illumina Hiseq4000 was used to complete the research. Overall design: 12 samples: samples of cell growth phase, curdlan-producing phase (normoxia) and curdlan-producing phase (micro-oxygen treated) in both Agrobacterium sp. ATCC 31749 wild strain and ΔfnrN strain were collectecd to extract mRNA. Each sample was treated in duplicate.

INSTRUMENT(S): Illumina HiSeq 4000 (Agrobacterium sp. ATCC 31749)

SUBMITTER: 王 永远  

PROVIDER: GSE92937 | GEO | 2017-12-27

SECONDARY ACCESSION(S): PRJNA359112

REPOSITORIES: GEO

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