Genomics

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Comprehensive immunophenotypic characterization of Burkitt-like lymphoma with 11q aberration by flow-cytometry


ABSTRACT: Recently, a subset of MYC-translocation negative aggressive B-cell lymphomas resembling Burkitt lymphoma (BL) characterized by proximal gains and distal losses in the long arm of chromosome 11 has been described. In the 2016 revision of the WHO classification these MYC-translocation negative lymphomas have been introduced as new provisional entity designated “Burkitt-like lymphoma with 11q aberration” (MNBLL 11q). Here, we show a comprehensive flow-cytometry analysis of 10 MNBLL 11q cases, well characterized genetically and pathologically. Twenty-three cases of MYC-positive BL, including three cases carrying both MYC rearrangements and 11q aberration, served as controls. All MNBLL 11q were CD20+/CD10+/BCL6+/BCL2- /MUM1- /MYC+/EBV negative , presented a high proliferation rate and showed a three-year overall survival (80%) similar to BL patients, with no recurrence after the end of treatment. MNBLL 11q immunophenotype was similar to that of MYC-positive BL without and with 11q, except for less frequent CD38higher expression (10% MNBLL 11q vs 91% MYC-positive BL, p<0.001), less frequent diminished CD45 expression (90% vs 23%, p=0.001), and CD16/CD56 co-expression (60% vs 0%, p<0.001). Our findings suggest subtle but important differences in MNBLL 11q immunophenotypes and MYC-positive BLs, which could not only aid in the differential diagnosis but also in the understanding of the pathogenesis of MNBLL 11q. Overall design: CytoSureTM Haematological Cancer and SNP Array (8x60k) (Oxford Gene Technology (OGT).The array has an average resolution of 68 Kb (SNP probe resolution 30Mb), but presents a resolution of 46.6 Kb in the 11q regions linked with hematological neoplasms. Normal DNA was pooled from normal individuals: 5 random women and 4 random men

INSTRUMENT(S): Agilent-036901 CytoSure Haematological Cancer + SNP 8x60k

ORGANISM(S): Homo Sapiens

SUBMITTER: Itziar Salaverria  

PROVIDER: GSE93002 | GEO | 2019-07-30

REPOSITORIES: GEO

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We previously described a subset of MYC translocation-negative aggressive B-cell lymphomas resembling Burkitt lymphoma, characterized by proximal gains and distal losses in chromosome 11. In the 2016 WHO classification, these MYC-negative lymphomas were recognized as a new provisional entity, 'Burkitt-like lymphoma with 11q aberration'. Here we present an immunophenotype analysis of Burkitt-like lymphomas with 11q aberration. Cells were acquired by fine needle aspiration biopsy from 10 young adu  ...[more]

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