Transcriptomics,Genomics

Dataset Information

35

Transcriptomic analysis of Mtb H37Rv and MtbΔwhiB4 on treatment to 10X Augmentin (AG) i.e. combination of 100 µg/ml of Amoxicillin and 8 µg/ml of Clavulanate and lower Augmentin doses at 1X and 5X


ABSTRACT: Amox-Clav combination downregulates the expression of WhiB4. Further, MtbΔwhiB4 survives better upon treatment with this combination. Therefore, to study the role of WhiB4 in regulating the response of Mtb to this combination, we carried out global transcriptome profiling of Wt Mtb and MtbΔwhiB4 strains after treatment. Overall design: Mtb H37Rv and MtbΔwhiB4 were grown till early log phase and treated with 10X Augmentin at 100 µg/ml of Amoxicillin and 8 µg/ml of Clavulanate for 6 h at 37 degree at 150rpm. Wildtype Mtb H37Rv were treated with 1X Augmentin (10 µg/ml of Amoxicillin and 8 µg/ml of Clavulanate), or 5X Augmentin (50 µg/ml of Amoxicillin and 8 µg/ml of Clavulanate) for 6 or 12 h at 37 degree at 150rpm. Experiments were done in duplicate, triplicate, tetraplicate or pentaplicate.

INSTRUMENT(S): UDSC M. tuberculosis H37Rv Validated Array

SUBMITTER: AMIT SINGH  

PROVIDER: GSE93091 | GEO | 2017-06-20

SECONDARY ACCESSION(S): PRJNA359935

REPOSITORIES: GEO

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Publications

Efficacy of β-lactam/β-lactamase inhibitor combination is linked to WhiB4-mediated changes in redox physiology of Mycobacterium tuberculosis.

Mishra Saurabh S   Shukla Prashant P   Bhaskar Ashima A   Anand Kushi K   Baloni Priyanka P   Jha Rajiv Kumar RK   Mohan Abhilash A   Rajmani Raju S RS   Nagaraja Valakunja V   Chandra Nagasuma N   Singh Amit A  

eLife 20170526


Mycobacterium tuberculosis (Mtb) expresses a broad-spectrum β-lactamase (BlaC) that mediates resistance to one of the highly effective antibacterials, β-lactams. Nonetheless, β-lactams showed mycobactericidal activity in combination with β-lactamase inhibitor, clavulanate (Clav). However, the mechanistic aspects of how Mtb responds to β-lactams such as Amoxicillin in combination with Clav (referred as Augmentin [AG]) are not clear. Here, we identified cytoplasmic redox potential and intracellula  ...[more]

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