Genomics

Dataset Information

0

Spemann organizer transcriptome induction by early β-Catenin, Wnt, Nodal and Siamois signals in Xenopus laevis


ABSTRACT: During Xenopus gastrulation, dorsal stabilization of β-Catenin at the earliest stage and subsequent target genes expression are critical for dorsal-ventral axis determination. However, many β-Catenin targets that mediate this process are still unkown. Here through RNA-seq analysis of β-Catenin knockdown embryos and self-regulating dorsal and ventral half embryos at early gastrula, we define an early β-Catenin gene signature that is downregulated by β-Catenin MO and enriched in dorsal gastrula tissues. This gene signature includes classic Spemann organizer genes, as well as other novel genes. Further analyses revealed that the early β-Catenin gene signature is positively correlated with LiCl treated, Wnt8 and Siamois mRNA-induced genes, consistent with their early role in dorsal-ventral axis formation. Our results also show that St 10.5 is the appropriate stage to uncover β-Catenin target genes that regulate dorsal-ventral patterning than St 9. Meanwhile, the multi-growth factor antagonist Cerberus inhibits part of the early β-Catenin gene signature and also controls the expression of a unique set of genes. Our findings provide new insight into the pivotal role of β-catenin in dorsal-ventral axis determination and can serve a fruitful resource for this field.

ORGANISM(S): Xenopus laevis

PROVIDER: GSE93195 | GEO | 2017/03/15

SECONDARY ACCESSION(S): PRJNA360282

REPOSITORIES: GEO

Similar Datasets

| E-GEOD-8990 | biostudies-arrayexpress
2007-11-01 | GSE8990 | GEO
2018-05-04 | GSE107424 | GEO
2021-01-06 | MODEL2002190001 | BioModels
2022-05-02 | GSE195806 | GEO
| E-MTAB-2617 | biostudies-arrayexpress
2016-06-20 | GSE75278 | GEO
2019-10-03 | PXD006781 | Pride
2023-07-08 | PXD035167 | JPOST Repository
| E-GEOD-3368 | biostudies-arrayexpress