Transcriptomics,Genomics

Dataset Information

42

Identification of a T cell signature associated with allergic diseases in Human


ABSTRACT: We wish to show that Th2A cells have a distinct gene expression profile compared to Th2 cells in allergic subjects Overall design: We sorted conventional Th1 cells (CXCR3+ CCR4- CCR6-), conventional Th17 cells (CCR6+), Th2A cells (CRTH2+ CD161+ CD49d+ CD27- CD45RB-) and conventional Th2+ cells (CRTH2+ CD161-) from the PBMC of 3 subject pools. Each pool consisted of cells from 2-3 allergic donors. Sorted TH subsets were stimulated for 6 hours with anti-CD3/CD28 beads (Life Technologies, STIM samples) or left unstimulated (CTRL samples) prior to extraction of RNA (RNeasy Mini kit; Qiagen).

INSTRUMENT(S): Illumina HumanHT-12 V4.0 expression beadchip

SUBMITTER: Scott Presnell  

PROVIDER: GSE93219 | GEO | 2017-10-05

SECONDARY ACCESSION(S): PRJNA360321

REPOSITORIES: GEO

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Publications


Allergen-specific type 2 helper T (TH2) cells play a central role in initiating and orchestrating the allergic and asthmatic inflammatory response pathways. One major factor limiting the use of such atopic disease-causing T cells as both therapeutic targets and clinically useful biomarkers is the lack of an accepted methodology to identify and differentiate these cells from overall nonpathogenic TH2 cell types. We have described a subset of human memory TH2 cells confined to atopic individuals t  ...[more]

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