Genomics

Dataset Information

165

The conserved protein Seb1 from fission yeast is recruited to the chromatin during active Pol II transcription


ABSTRACT: The protein Seb1 from fission yeast contains a conserved CTD-interacting domain (CID) with which it can bind to phosphorylated forms of the Pol II C-terminal domain (CTD) during active transcription. It mainly interacts with Ser2P-CTD but also with Ser5P-CTD. Here, we show the recruitment profile of the protein to chromatin using ChIP-Seq which is mediated mainly via binding to the Pol II-CTD. In addition, it can also interact with nascent RNA via its RNA recognition motif (RRM) domain. Overall design: Two inputs and two immunoprecipitated Seb1-TAP samples were used to perform ChIP-seq. An untagged control (called WT) is also provided.

INSTRUMENT(S): Ion Torrent PGM (Schizosaccharomyces pombe)

SUBMITTER: Beth Watts  

PROVIDER: GSE93342 | GEO | 2017-02-14

SECONDARY ACCESSION(S): PRJNA360641

REPOSITORIES: GEO

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Publications

The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA.

Wittmann Sina S   Renner Max M   Watts Beth R BR   Adams Oliver O   Huseyin Miles M   Baejen Carlo C   El Omari Kamel K   Kilchert Cornelia C   Heo Dong-Hyuk DH   Kecman Tea T   Cramer Patrick P   Grimes Jonathan M JM   Vasiljeva Lidia L  

Nature communications 20170403


Termination of RNA polymerase II (Pol II) transcription is an important step in the transcription cycle, which involves the dislodgement of polymerase from DNA, leading to release of a functional transcript. Recent studies have identified the key players required for this process and showed that a common feature of these proteins is a conserved domain that interacts with the phosphorylated C-terminus of Pol II (CTD-interacting domain, CID). However, the mechanism by which transcription terminati  ...[more]

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