Transcriptomics,Genomics

Dataset Information

23

NIA15k screen. Pax5 +/+, Pax5 -/-, and Rag1-/- pro B cell lines.


ABSTRACT: The transcription factor Pax5 is essential for B cell commitment in the mouse, where it represses lineage-inappropriate gene expression, while simultaneously activating the B cell gene expression program. We have performed a global gene expression screen of wild type and Pax5-deficient pro-B cells in an attempt to identify the crucial Pax5 targets in early B-lymphopoiesis. We have also included Rag1-/- and wild type (+/+) proB cells starved of the cytokine IL-7 for 4 hours as controls. Rag1-/- proB cells are incapable of further differentiation due to an absence of immunoglobulin recombination and IL-7 is the major cytokine regulating proB cell growth. Keywords: comparison of genetically modified cell-lines Overall design: Twelve arrays were used to compare a single Pax5+/+ proB cell line grown in the presence or abscence of IL-7 for 4 hours. 3 independently derived Pax5-/- proB cells lines and a single Rag1-/- pro B cell line were also analysed. cDNA were hybridised in a saturated loop design using dye swaps for each comparison, such that all samples were compared twice.

INSTRUMENT(S): NIA 15K Mouse (AGRF Nov 2001)

SUBMITTER: Stephen L Nutt   

PROVIDER: GSE9345 | GEO | 2007-10-26

SECONDARY ACCESSION(S): PRJNA103033

REPOSITORIES: GEO

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Publications

Identification of Pax5 target genes in early B cell differentiation.

Pridans Clare C   Holmes Melissa L ML   Polli Matthew M   Wettenhall James M JM   Dakic Aleksandar A   Corcoran Lynn M LM   Smyth Gordon K GK   Nutt Stephen L SL  

Journal of immunology (Baltimore, Md. : 1950) 20080201 3


The transcription factor Pax5 is essential for B cell commitment in the mouse, where it represses lineage-inappropriate gene expression while simultaneously activating the B cell gene expression program. In this study we have performed a global gene expression screen of wild-type and Pax5-deficient pro-B cells in an attempt to identify the crucial Pax5 targets in early B lymphopoiesis. These studies have identified 109 Pax5 targets comprising 61% activated and 39% repressed genes. Interestingly,  ...[more]

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