Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression and alternative splicing profiles of BEZ235 treated Ewing Sarcoma cells

ABSTRACT: TC71 cells treated either with BEZ235 or DMSO

ORGANISM(S): Homo sapiens

PROVIDER: GSE93579 | GEO | 2018/02/14

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Ewing Sarcoma cell lines treated with mithramycin

Project description:The study aims to define gene expression changes associated with mithramycin treatment of Ewing Sarcoma cell lines. The data consist of 12 arrays. Two cell lines, TC71 and TC32, were treated with solvent control or with mithramycin, and RNA was extracted at 6 hours. Three biological replicates per cell line/treatment.

2011-04-18 | E-GEOD-25127 | biostudies-arrayexpress

Human basal-like breast cancer cell line HCC1143 treated with BET inhibitor JQ1 in combination with MEK inhibitor Trametinib or PI3K/mTOR inhibitor BEZ235

Project description:The goal of this experiment was to understand the changes in gene expression in the human basal-like breast cancer cell line HCC1143 following treatment with the MEK inhibitor Trametinib (T), PI3K/mTOR inhibitor BEZ235 (B), the BET inhibition JQ1 (JQ), Trametinib + JQ1 (TJ), or BEZ235 + JQ1(BJ), compared to a DMSO control (D). Samples were treated for 72hr and run in triplicate.

2017-05-30 | GSE82032 | GEO

Influence of BEZ235 and MYC on gene expression in SW620 cells

Project description:SW620 were transfected with siMYC or siCTR at time point 0h. 24h later cells were splitter 1:2, additional 24 h later (48h after transfection) cells were treated with BEZ235 (200nM) or DMSO. Cells were harvested after additional 24h (72h after siRNA transfection; 24h BEZ235 treatment.

2015-08-27 | E-MTAB-2882 | biostudies-arrayexpress

Expression profiling of Ewing sarcoma cell lines exposed to 5nM Pladienolide B (PlaB) for 24 hours

Project description:Identification of genes and pathways altered by PlaB, a bacterial natural product that acts as a spliceosome modulator by targeting the SF3b subunit of the spliceosome SKNMC, TC32, TC71, and RD-ES Ewing sarcoma cell lines were treated with 0.1% (v/v) DMSO vehicle or 5nM PlaB for 24 hours. Three samples in each group were analyzed.

2016-01-15 | E-GEOD-67548 | biostudies-arrayexpress

Single cell transcriptomics of Ewing Sarcoma cell lines

Project description:We report single cell gene expression profiles for Ewing Sarcoma cell lines CHLA9, CHLA10, and TC71. Cell lines were harvested and prepared as single cell suspensions with high viability. Library prep was performed with the 10X Genomics v3.1 3' kit. All samples were sequenced on the illumina HiSeq 3000 with a 28+8+100 configuration. CHLA9 and CHLA10 libraries were re-sequenced on the Illumina NovaSeq with a 28+8+91 configuration. Counts were processed with cellranger v3.1.0. Both the raw data and the cellranger output are provded.

2020-04-11 | GSE146221 | GEO

Identification of potential miR-31 target-genes in Ewing Sarcoma

Project description:We identified miR-31 as strongest downregulated miRNA in the bone tumor Ewing sarcoma. To identify miR-31 targets in ES genome-wide gene expression profiles of TC71 cells transfected with miR-31 mimics or negative control (Life Technologies) generated in three independent experiments (a, b, c). Total RNA was isolated from cells after 72 h incubation in serum-reduced medium (1% FCS) and three transfections. Fifteen upregulated and 14 downregulated genes were detected with a 2 fold change cut-off (FDR <0.05).

2012-10-23 | E-MTAB-1334 | biostudies-arrayexpress

Expression profiling of Ewing sarcoma samples

Project description:Expression profiling of Ewing sarcoma samples in the frame of the CIT program from the french Ligue Nationale Contre le Cancer (http://cit.ligue-cancer.net). STAG2 loss-of-function mutation is the most frequent secondary genetic alteration in Ewing sarcoma, an aggressive bone tumor driven by the chimeric EWSR1-FLI1 transcription factor. STAG2 encodes an integral member of the cohesin complex, a ring-shaped multi-protein structure, which is essential to shape the architecture and expression of the genome with CTCF. Combining this cohort with our previously published series (GSE34620), we show that a signature of commonly downregulated genes upon STAG2 mutation in A673 and TC71 and linked to at least one EWSR1-FLI1 bound GGAA microsatellite enhancer chain element inferred form H3K27ac HiChIP predict poor overall survival in Ewing sarcoma patients.

2021-04-29 | GSE142162 | GEO

miR-130b drives activation of a metastatic CDC42-PAK1-AP1 positive feedback loop in Ewing sarcoma by directly targeting Arhgap1

Project description:Analysis of miR-130b regulated genes in TC71 Ewing Sarcoma cells. The hypothesis tested being that overexpression of miR-130b increases metastasis in Ewing Sarcoma.

2016-06-21 | GSE83548 | GEO

Knowk-down of EWSR1-FLI1 in Ewing sarcoma cell lines (RNA-seq)

Project description:EWSR1-FLI1 is a chimeric transcription factor resulting from the pathognomonic translocation present in Ewing sarcoma cells. Here, we silenced EWSR1-FLI1 in different Ewing sarcoma cell lines. RNA from SKNMC, TC71 and MHH-ES1 cells was extracted 96h post transfection (siCT or siEWSR1-FLI1) or prior doxycycline (day 0) and 7 days after inducing silencing of EWSR1-FLI1 with doxycycline in ASP14 cells. RNA-seq was performed for all conditions.

2022-05-09 | GSE164372 | GEO