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Genome wide gene expression analysis of differential fetal cotyledons

ABSTRACT: In an attempt to unveil the molecular mechanisms of fetal-maternal interactions during early pregnancy, we have performed the pregnancy induced gene expression analysis in the gravid uteri of fetal cotyledons using the Affymetrix GeneChip Genome Array. In this study, we have performed the microarray analysis of the RNA isolated from fetal cotyledon tissue of Bubalus bubalis on less than 45 day of pregnancy. We have identified total of 8587 differentially expressed genes with significance p ≤ 0.05 in four biological replicates. Bioinformatics analysis of microarray data reveal the cluster of genes involved at the placentome level for various functions such as fetus attachment, transport of nutrition, and immune response. Interestingly, we have identified multiple isoforms of pregnancy associated glycoproteins (PAGs) which were highly up regulated with average fold change of 472.93 in pregnant buffaloes. It was observed the genes responsible for increased immune response were down regulated, suggesting an immune suppressive environment which is required to adapt the semiallogenic fetus for successful pregnancy. We have identified the genes and pathways involved during embryo development and the cluster of genes involved in fetal-maternal interaction, attachment, nourishment of fetus, and immune response. The present study will provide future directions for developing pregnancy diagnosis kit in livestock which is highly necessary for increasing the reproductive efficiency of buffaloes.

ORGANISM(S): Bos taurus

PROVIDER: GSE93874 | GEO | 2017/12/30

SECONDARY ACCESSION(S): PRJNA362632

REPOSITORIES: GEO

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regulatory T cells in human pregnancy

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