BL-Hi-C is an efficient and sensitive approach for capturing structural and regulatory chromatin interactions

ABSTRACT: We propose a novel in situ Hi-C method named Bridge-Linker-Hi-C (BL-Hi-C) for structural and regulatory chromatin interactions capture by restriction enzyme targeting and two-step proximity ligation. It improves the sensitivity and specificity for active chromatin loop detection and can reveal a better enhancer-promoter regulatory architecture than conventional method at a lower sequencing depth and with a simpler protocol.

ORGANISM(S): Homo sapiens

PROVIDER: GSE93921 | GEO | 2018/01/23

REPOSITORIES: GEO