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Expression profiling of severe growth abortion (SGA) in triploid hybrids crossed between tetraploid wheat and Aegilops umbellulata.

ABSTRACT: We have employed whole genome microarray expression profiling as a discovery platform to identify genes to alter the transcript accumulation levels in SGA plants, which are triploid hybrids crossed between tetraploid wheat and a diploid wheat relative Aegilops umbellulata. Of the up-regulated genes, cell death and carboxy-lyase activity-related genes were the most frequently found in crown tissues of SGA plants. On the other hand, photosynthesis-related genes were highly down-regulated in crown tissues of SGA plants. In addition, transcript accumulation levels of shoot apical meristem (SGA)-related genes such as knotted-1 like homeobox (Knox) genes were also repressed in SGA plants. The microarray analysis strongly suggests that an autoimmune response-like reaction might be triggered by intergenomic incompatibility between the AB and U genomes in SGA. In addition, SAM-related and cell cycle-related genes were dramatically down-regulated in crown tissues of SGA, indicating that abnormalities of SAM are associated with the abnormal growth phenotypes in SGA.

ORGANISM(S): Triticum aestivum

PROVIDER: GSE93943 | GEO | 2017/01/24

SECONDARY ACCESSION(S): PRJNA362950

REPOSITORIES: GEO

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Expression profiling of severe growth abortion (SGA) in triploid hybrids crossed between tetraploid wheat and Aegilops tauschii.

Project description:We have employed whole genome microarray expression profiling as a discovery platform to identify genes to alter the transcript accumulation levels in SGA lines, which are triploid hybrids crossed between tetraploid wheat and diploid wheat progenitor Aegilops tauschii. Of the up-regulated genes, defense-related genes were most frequently found in crown tissues of SGA line. On the other hand, photosynthesis-related genes were highly down-regulated in crown tissues of SGA line. In addition, transcript accumulation levels of shoot apical meristem (SGA)-related genes such as knotted-1 like homeobox (Knox) genes were also repressed in SGA line. To validate the microarray data, RT-PCR and quantitative RT-PCR analyses for 23 selected genes were performed in total. Of the examined genes, 20 genes (87%) of expression pattern were consistent with the microarray data. The microarray analysis strongly suggests that an autoimmune response-like reaction might be triggered by intergenomic incompatibility between the AB and D genomes in SGA. In addition, SAM-related and cell cycle-related genes were dramatically down-regulated in crown tissues of SGA, indicating that abnormalities of SAM are associated with the abnormal growth phenotypes in SGA. Expression patterns were compared between the two triploid hybrids showing the wild-type phenotype (as a reference) and severe growth abortion. Total RNA samples were isolated from the 3-week-old crown tissues. Two independent experiments were conducted in each exprement.

2011-11-01 | E-GEOD-33357 | biostudies-arrayexpress

Dysfunction of mitotic cell division at shoot apices triggered severe growth abortion in interspecific hybrids between tetraploid wheat and Aegilops tauschii Coss.

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Expression profiling of grass-clump dwarf in synthetic hexaploids from triploid hybrids crossed between tetraploid wheat and Aegilops tauschii

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Transcriptional profiling during OSH1 induction

Project description:In flowering plants, knotted1-like homeobox (KNOX) transcription factors play crucial roles in establishment and maintenance of the shoot apical meristem (SAM), from which aerial organs such as leaves, stems and flowers initiate. We report that a rice (Oryza Sativa) KNOX gene Oryza sativa homeobox1 (OSH1) represses the brassinosteroid (BR) phytohormone pathway through activation of BR catabolism genes. Inducible overexpression of OSH1 caused brassinosteroid insensitivity, whereas loss-of-function showed a BR-overproduction phenotype. Genome-wide identification of loci bound and regulated by OSH1 revealed hormonal and transcriptional regulation as the major function of OSH1. Among these targets, BR catabolism genes CYP734A2, CYP734A4 and CYP734A6 were rapidly up-regulated by OSH1-induction. Furthermore, RNAi knockdown plants of CYP734A genes arrested growth of the SAM and mimicked some osh1 phenotypes. Thus, we suggest that local control of BR levels by KNOX genes is a key regulatory step in SAM function.

2014-08-07 | E-MTAB-4316 | biostudies-arrayexpress

Expression profiling of cold-induced hybrid necrosis in triploid hybrids crossed between tetraploid wheat and Aegilops tauschii.

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Project description:We have employed whole genome microarray expression profiling as a discovery platform to identify genes to alter the transcript accumulation levels in hybrid necrosis-showing plants, which are triploid hybrids crossed between tetraploid wheat and diploid wheat progenitor Aegilops tauschii. Of the up-regulated genes, defense-related genes were most frequently found, whereas photosythesis-related genes down-regulated in the type I necrosis line. To validate the microarray data, RT-PCR and quantitative RT-PCR analyses for 22 selected genes were performed. Of the examined 15 up-regulated and 7 down-regulated genes, the expression pattern of only one down-regulated gene was inconsistent with the microarray data. Together with cytological analysis of the necrotic tissues, the microarray analysis strongly suggests that an autoimmune response might be triggered by intergenomic incompatibility between the AB and D genomes in type I necrosis, and that genetically programmed cell death could be regarded as a hypersensitive response-like cell death similar to that observed in Arabidopsis intraspecific and Nicotiana interspecific hybrids.

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2014-08-12 | E-GEOD-59640 | biostudies-arrayexpress

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