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Host-parasite transcriptomics during immunostimulant enhanced rejection of salmon lice Lepeophtheirus salmonis by Atlantic salmon Salmo salar

ABSTRACT: We investigate the effect of a functional feed for immunostimulation (peptidoglycan extract from bacterial cell wall with nucleotide formulation) on L. salmonis infection levels on Atlantic salmon Salmo salar, and on host and parasite gene expression profiles. Atlantic salmon smolts (~95 g) were fed a control diet, or a low or high dose immunostimulant diet, and then exposed to L. salmonis copepodids in three subsequent exposures. The transcriptome of salmon lice late in the infection attached to either the low dose diet or control diet hosts were compared using a 38K oligonucleotide microarray.

ORGANISM(S): Lepeophtheirus salmonis

PROVIDER: GSE93998 | GEO | 2017/02/26

SECONDARY ACCESSION(S): PRJNA363024

REPOSITORIES: GEO

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Sex-Biased Gene Expression and Sequence Conservation in Pacific and Atlantic Salmon Lice (Lepeophtheirus salmonis)

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Sex-Biased Gene Expression and Sequence Conservation in Pacific and Atlantic Salmon Lice (Lepeophtheirus salmonis)

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Enhanced transcriptomic responses in the Pacific salmon louse Lepeophtheirus salmonis oncorhynchi to the non-native Atlantic Salmon Salmo salar suggests increased parasite fitness

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Transcriptomic responses to emamectin benzoate in Pacific and Atlantic Canada salmon lice Lepeophtheirus salmonis with differing levels of drug resistance

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2014-11-17 | E-GEOD-56024 | biostudies-arrayexpress

Dietary phytochemicals modulate skin gene expression and reduce lice counts after infection in Atlantic salmon

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Transcriptomic profiling in fins of Atlantic salmon parasitized with sea lice: Determining the differentially expressed transcripts in fins of lice-infected salmon at 8 days post-infection

Project description:Sea lice (e.g., Lepeophtheirus salmonis) are parasites causing costly disease outbreaks in salmon farming globally. Despite the currently available controlling practices, molecular insights into the parasite-induced host immune responses and host-pathogen interaction will provide the basis for improved and innovative treatment strategies. We investigated the early transcriptomic responses in the fins of Atlantic salmon parasitized with sea lice at the chalimus stage (8 days post-infection). Fin tissue collected from non-infected (PRE) control and at (ATT) and adjacent (ADJ) to chalimus-attachment sites from infected fish were used in profiling the global gene expression using a 44K microarray platform. Microarray analyses indicated that global transcriptomic changes resulted in 6,568 differentially expressed probes (DEPs, FDR < 5%) that include 1928 shared DEPs between ATT and ADJ compared to PRE. A direct comparison of ATT vs. ADJ revealed 90 DEPs, all of which were up-regulated in ATT.

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