Genomics

Dataset Information

161

Analysis of nonsense-mediated mRNA decay in daf-2 mutants


ABSTRACT: To exmine the role of nonsense-mediated mRNA decay process in the longevity regulation of daf-2 mutants, we sequenced transcriptomes from day 1 adult Caenorhabditis elegans: Bristol N2 (wild-type), and smg-2(qd101), daf-2(e1370) and smg-2(qd101); daf-2(e1370) mutants. Overall design: Determination of transcript levels in N2 (wild-type), smg-2(qd101), daf-2(e1370), and smg-2(qd101); daf-2(e1370) animals by using deep sequencing, in duplicate

INSTRUMENT(S): Illumina HiSeq 2500 (Caenorhabditis elegans)

SUBMITTER: Seung-Jae V Lee  

PROVIDER: GSE94077 | GEO | 2017-03-17

SECONDARY ACCESSION(S): PRJNA368736

REPOSITORIES: GEO

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Publications


Long-lived organisms often feature more stringent protein and DNA quality control. However, whether RNA quality control mechanisms, such as nonsense-mediated mRNA decay (NMD), which degrades both abnormal as well as some normal transcripts, have a role in organismal aging remains unexplored. Here we show that NMD mediates longevity in C. elegans strains with mutations in daf-2/insulin/insulin-like growth factor 1 receptor. We find that daf-2 mutants display enhanced NMD activity and reduced leve  ...[more]

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