MastomysMouseMucosa
Ontology highlight
ABSTRACT:
ORGANISM(S): Mastomys natalensis Mus musculus
PROVIDER: GSE941 | GEO | 2004/01/07
SECONDARY ACCESSION(S): PRJNA87979
REPOSITORIES: GEO
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Project description:Analysis of gene expression in an enriched ECL cell population from the agstric mucosa of Mastomys treated with Loxtidine for 8-16 weeks, and from an animal that spontaneously developed an ECL cell carcinoid Keywords = ECL Mastomys Affymetrix Carcinoid Gene Keywords: other
2004-01-07 | GSE942 | GEO
Project description:Gene expression analysis of stomach ECL cells and mixed gastric fundic epithelial cells Keywords: Gastric ECL cells, Subtractive Hybridization, Comparative genomic hybridization
2006-01-26 | GSE3518 | GEO
Project description:Gastric ECL cells and parietal (PC) cells in normal conditions and gastric ECL cells after 24 hrs fasting were purified using a combination of counter-flow elutriation, nycodenz gradient and FACS based on acridine orange labeling of histamine containing vesicles (ECL cells) or autofluorescense (PC cells) to homogeneity. cRNA probes from this purified cell suspensions and from mixed gastric homogenates were hybridized to whole rat genome expression oligonucleotide microarrays. We imported each channel separately (normalized intensity of Cy3 labeled samples = gProcessedSignal and of Cy5 labeled samples = rProcessedSignal) from the original Agilent Feature Extraction data files (submitted as raw data) after slide scanning from all slides into a Genespring 7.3 microarray database warehouse. Genespringâs built in statistical module was used to compare all channels to each other. The normalization used was against specific samples (the 5 channels of Gastric mucosal scrapings). Genespring identifies genes differentially expressed in the ECL cell samples and the parietal samples and also calculates the significance of difference (p-value). Experiment Overall Design: ECL cells: free access to food and water (250 gm Sprague Dawley Rat) Experiment Overall Design: PC cells: free access to food and water (250 gm Sprague Dawley Rat) Experiment Overall Design: ECL cells: 24 hrs fasting, free access to water (250 gm Sprague Dawley Rat) Experiment Overall Design: Gastric mucosa scrapes: free access to food and water (250 gm Sprague Dawley Rat) Experiment Overall Design: Fasting gastric mucosa scrapes: 24 hrs fasting, free access to water (250 gm Sprague Dawley Rat) Experiment Overall Design: Three independent cell purifications, total RNA isolations and at least one dye swap per sample set (3).
2008-06-14 | E-GEOD-6204 | biostudies-arrayexpress
Project description:Previous studies have shown that treatment with the somatostatin analogue octreotide LAR causes regression of gastric ECL-cell carcinoids in patients with hypergastrinaemia due to chronic atrophic gastritis, reducing both the number and size of tumours. The main objective of the present study was to examine the molecular mechanisms behind the antiproliferative effect of octreotide in the oxyntic mucosa on a genome wide scale. Female Sprague-Dawley rats were dosed with octreotide LAR and control group were given the LAR vehicle for 21 days. Serum gastrin levels were measured and tissue samples for histology and RNA extraction collected from the oxyntic mucosa. Histomorphological examination showed a significant decrease in the number of gastric glands, cells per gland and length of glands, indicating a negative effect of octreotide on growth of the oxyntic mucosa. Further immunohistochemical studies showed a tendency towards increased apoptosis and decreased proliferation in the group receiving octreotide. Affymetrix GeneChip microarrays were used to detect differentially expressed genes. Many regulated genes could be related to regulation of apoptosis, fewer to proliferation, and the largest group of regulated genes was transcriptional factors several of which may be involved in regulation of growth. Control studies using quantitative real-time RT-PCR showed a high degree of consistency of the microarray results. In conclusion, octreotide exerts a negative effect on growth of the oxyntic mucosa, and extensive gene expression changes relevant to growth regulation can be detected. Keywords: regulation, proliferation, Affymetrix GeneChip, qRT-PCR, immunohistochemistry.
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Project description:Gastric ECL cells and parietal (PC) cells in normal conditions and gastric ECL cells after 24 hrs fasting were purified using a combination of counter-flow elutriation, nycodenz gradient and FACS based on acridine orange labeling of histamine containing vesicles (ECL cells) or autofluorescense (PC cells) to homogeneity. cRNA probes from this purified cell suspensions and from mixed gastric homogenates were hybridized to whole rat genome expression oligonucleotide microarrays. We imported each channel separately (normalized intensity of Cy3 labeled samples = gProcessedSignal and of Cy5 labeled samples = rProcessedSignal) from the original Agilent Feature Extraction data files (submitted as raw data) after slide scanning from all slides into a Genespring 7.3 microarray database warehouse. Genespring’s built in statistical module was used to compare all channels to each other. The normalization used was against specific samples (the 5 channels of Gastric mucosal scrapings). Genespring identifies genes differentially expressed in the ECL cell samples and the parietal samples and also calculates the significance of difference (p-value). Keywords: rat whole genome expression microarray analysis (transcriptome)
2006-11-22 | GSE6204 | GEO
Project description:To clarify the molecular pathogenesis of DFL pathogenesis using GEP. DFL shares characteristics of both NFL and MALT lymphoma. 55 total samples were examined; 10 DFL, 18 NFL, 10 gastric MALT lymphoma, 5 normal duodenal mucosa, 8 nodal reactive lymphoid hyperplasia, and 4 normal gastric mucosa.
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Project description:Analysis of gene expression in gastric mucosa from Mastomys treated for different time periods with Loxtidine
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2007-05-03 | GSE4810 | GEO
Project description:Affymetrix RG U34A analysis of renal gene expression in Wistar-derived rat strains Keywords: repeat sample
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