Transcriptomics,Genomics

Dataset Information

29

Expression data from KEAP1 overexpression and NRF2 siRNA knockdown of A549 NSCLC cell


ABSTRACT: KEAP1 overexpressed and NRF2 siRNA knockdown A549 NSCLC cells were used to identify downstream genes of NRF2 pathway separately and by combinatorial analysis. We used triplicate microarrays of transfected A549 cells with mKeap1-GFP for overexpression, siRNAs targeting NRF2 for knockdown and siGFP as control respectively. As a result, we identified several genes which are involved in cancer metabolic functions in these cells. We used microarrays to identify the gene downregulated in both KEAP1 overexpressed and NRF2 siRNA knockdown A549 NSCLC cells and found a subset of downregulated genes which are involved in metabolic functions. Overall design: We divided microarrays into three groups. One group with A549 cells that were stably transfected with mKeap1-GFP construct for overexpression, second group with knockdown of NRF2 with specific siRNA and third group contains siGFP transfected control cells. Triplicates of both KEAP1 overexpression and NRF2 siRNA knockdown groups were analyzed and compared with control microarray data.

INSTRUMENT(S): [PrimeView] Affymetrix Human Gene Expression Array

SUBMITTER: AKHILESHWAR NAMANI  

PROVIDER: GSE94393 | GEO | 2017-08-02

SECONDARY ACCESSION(S): PRJNA369558

REPOSITORIES: GEO

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Publications

NRF2-regulated metabolic gene signature as a prognostic biomarker in non-small cell lung cancer.

Namani Akhileshwar A   Cui Qin Qin QQ   Wu Yihe Y   Wang Hongyan H   Wang Xiu Jun XJ   Tang Xiuwen X  

Oncotarget 20170718 41


Mutations in Kelch-like ECH-associated protein 1 (KEAP1) cause the aberrant activation of nuclear factor erythroid-derived 2-like 2 (NRF2), which leads to oncogenesis and drug resistance in lung cancer cells. Our study was designed to identify the genes involved in lung cancer progression targeted by NRF2. A series of microarray experiments in normal and cancer cells, as well as in animal models, have revealed regulatory genes downstream of NRF2 that are involved in wide variety of pathways. Spe  ...[more]

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