Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Context-dependent miR-204 and miR-211 affect the biological properties of amelanotic and melanotic melanoma cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE94477 | GEO | 2017/03/26

SECONDARY ACCESSION(S): PRJNA369800

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Context-dependent miR-204 and miR-211 affect the biological properties of amelanotic and melanotic melanoma cells.

Project description: Not available

| S-EPMC5421939 | biostudies-literature

Context-dependent miR-204 and miR-211 affect the biological properties of amelanotic and melanotic melanoma cells [array]

Project description:Despite increasing amounts of experimental evidence depicting the involvement of non-coding RNAs in cancer, the study of BRAFV600E-regulated genes has thus far focused mainly on protein-coding ones. Here, we identify and study the microRNAs that BRAFV600E regulates through the ERK pathway. By performing small RNA sequencing on A375 melanoma cells and a vemurafenib- resistant clone that was taken as negative control, we discover miR-204 and miR-211 as the miRNAs most induced by vemurafenib. We also demonstrate that, although belonging to the same family, these two miRNAs have distinctive features. miR-204 is under the control of STAT3 and its expression is induced in amelanotic melanoma cells, where it acts as an effector of vemurafenib’s anti-motility activity by targeting AP1S2. Conversely, miR-211, a known transcriptional target of MITF, is induced in melanotic melanoma cells, where it targets EDEM1 and consequently impairs the degradation of TYROSINASE (TYR) through the ER-associated degradation (ERAD) pathway. In doing so, miR-211 serves as an effector of vemurafenib’s pro-pigmentation activity. We also show that such an increase in pigmentation in turn represents an adaptive response that needs to be overcome using appropriate inhibitors in order to increase the ef cacy of vemurafenib. In summary, we unveil the distinct and context-dependent activities exerted by miR-204 family members in melanoma cells. Our work challenges the widely accepted “same miRNA family = same function” rule and provides a rationale for a novel treatment strategy for melanotic melanomas that is based on the combination of ERK pathway inhibitors with pigmentation inhibitors.

2017-03-26 | GSE94476 | GEO

Context-dependent miR-204 and miR-211 affect the biological properties of amelanotic and melanotic melanoma cells [miRNA-Seq]

2017-03-26 | GSE94423 | GEO

Context-dependent miR-204 and miR-211 affect the biological properties of amelanotic and melanotic melanoma cells

Project description:Context-dependent miR-204 and miR-211 affect the biological properties of amelanotic and melanotic melanoma cells

| PRJNA369800 | ENA

Biological role of miR-204 and miR-211 in melanoma.

Project description: Not available

| S-EPMC6142896 | biostudies-other

miR-204-5p and miR-211-5p Contribute to BRAF Inhibitor Resistance in Melanoma.

Project description: Not available

| S-EPMC5815895 | biostudies-literature

miR-204-5p and miR-211-5p contribute to BRAF inhibitor resistance in melanoma

Project description:Purpose: Identification of miRNAs that enable resistance to BRAF inhibitors in melanoma suggests a mechanism-based strategy to limit resistance and improve clinical outcomes. Methods: We generated A375 melanoma cells resistant to Vemurafenib (VMF) with the goal of investigating changes in miRNA expression patterns that might contribute to resistance. Results: Increased expression of miR-204-5p and miR-211-5p occurring in VMF-resistant cells was determined to impact VMF response.

2018-01-18 | GSE107576 | GEO

Gene expression profile analysis of inducible melanotic and amelanotic melanoma in mice

Project description:We analyzed an inducible melanoma model in TiRP Ink4a/ArfF/F B10.D2 mice based on the conditional deletion in melanocytes of two tumor suppressor genes INK4a/Arf coupled to the expression of the oncogene H-rasG12V and a known tumor antigen (Hujibers et al. Cancer Res. 66:3278,2006; Soudja et al. Cancer Res. 70:3515-3525,2010). About 40% of these mice develop melanomas. Some tumors are heavily pigmented melanotic melanoma and grow slowly (hereafter referred to as “Mela”), but most of the induced tumors are nonpigmented amelanotic melanomas which are more aggressive (hereafter referred to as “Amela”). Gene expression profiles of the two types of tumors and of Amela-derived cell lines established in vitro were analyzed to identify the transcriptional signatures of these two types of tumors (including stromal components and infiltrating cells), as well as that intrinsic to Amela-tumor cells. 4 Amela and 4 Mela ex-vivo tumors were compared in a dye-swap experiment (8 chips). 4 Amela and 4 Mela ex-vivo tumors were compared to healthy skin (CTRL) (8 chips). 4 Amela ex-vivo tumors and Amela cell lines were compared in a dye-swap experiment (8 chips).

2013-06-26 | E-GEOD-29304 | biostudies-arrayexpress

Gene expression profile analysis of inducible melanotic and amelanotic melanoma in mice

2013-06-26 | GSE29304 | GEO

The microRNAs miR-204 and miR-211 maintain joint homeostasis and protect against osteoarthritis progression.

Project description: Not available

| S-EPMC6599052 | biostudies-literature